Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54795..55438 Replicon plasmid pCY814036-iucA
Accession NZ_CP093152
Organism Klebsiella pneumoniae strain CY814036

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MMZ10_RS26745 Protein ID WP_001044770.1
Coordinates 55022..55438 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MMZ10_RS26740 Protein ID WP_001261282.1
Coordinates 54795..55025 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMZ10_RS26705 (MMZ10_26705) 49804..50979 + 1176 Protein_48 IS3 family transposase -
MMZ10_RS26710 (MMZ10_26710) 51026..51241 + 216 WP_129586912.1 hypothetical protein -
MMZ10_RS26715 (MMZ10_26715) 51361..52146 - 786 WP_050485696.1 site-specific integrase -
MMZ10_RS26720 (MMZ10_26720) 52192..52713 - 522 WP_032448294.1 hypothetical protein -
MMZ10_RS26725 (MMZ10_26725) 52771..53163 - 393 WP_074422814.1 hypothetical protein -
MMZ10_RS26730 (MMZ10_26730) 53272..54216 - 945 WP_032448293.1 hypothetical protein -
MMZ10_RS26735 (MMZ10_26735) 54416..54838 - 423 WP_200853072.1 hypothetical protein -
MMZ10_RS26740 (MMZ10_26740) 54795..55025 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MMZ10_RS26745 (MMZ10_26745) 55022..55438 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MMZ10_RS26750 (MMZ10_26750) 55512..57074 + 1563 WP_004206609.1 AAA family ATPase -
MMZ10_RS26755 (MMZ10_26755) 57059..58081 + 1023 WP_000361404.1 helicase UvrD -
MMZ10_RS26760 (MMZ10_26760) 58626..59534 + 909 WP_137877090.1 HNH endonuclease -
MMZ10_RS26765 (MMZ10_26765) 59720..60070 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / blaLAP-2 / qnrS1 iutA / iucD / iucC / iucB / iucA 1..257343 257343
- flank IS/Tn - - 60235..61215 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T237715 WP_001044770.1 NZ_CP093152:55022-55438 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T237715 NZ_LR882500:c446110-445517 [Mycobacterium tuberculosis variant microti]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT237715 WP_001261282.1 NZ_CP093152:54795-55025 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT237715 NZ_LR882500:c446528-446139 [Mycobacterium tuberculosis variant microti]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References