Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5345822..5346447 Replicon chromosome
Accession NZ_CP093151
Organism Klebsiella pneumoniae strain CY814036

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MMZ10_RS26095 Protein ID WP_002882817.1
Coordinates 5345822..5346205 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MMZ10_RS26100 Protein ID WP_004150355.1
Coordinates 5346205..5346447 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMZ10_RS26080 (5343188) 5343188..5344090 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
MMZ10_RS26085 (5344087) 5344087..5344722 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
MMZ10_RS26090 (5344719) 5344719..5345648 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MMZ10_RS26095 (5345822) 5345822..5346205 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MMZ10_RS26100 (5346205) 5346205..5346447 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MMZ10_RS26105 (5346652) 5346652..5347569 + 918 WP_004901674.1 alpha/beta hydrolase -
MMZ10_RS26110 (5347583) 5347583..5348524 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MMZ10_RS26115 (5348569) 5348569..5349006 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MMZ10_RS26120 (5349003) 5349003..5349863 - 861 WP_002882807.1 virulence factor BrkB family protein -
MMZ10_RS26125 (5349857) 5349857..5350456 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T237714 WP_002882817.1 NZ_CP093151:c5346205-5345822 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T237714 NZ_LR882500:365270-365695 [Mycobacterium tuberculosis variant microti]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT237714 WP_004150355.1 NZ_CP093151:c5346447-5346205 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT237714 NZ_LR882500:365052-365273 [Mycobacterium tuberculosis variant microti]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References