Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4733932..4734742 Replicon chromosome
Accession NZ_CP093151
Organism Klebsiella pneumoniae strain CY814036

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MMZ10_RS23245 Protein ID WP_004178461.1
Coordinates 4733932..4734465 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag MMZ10_RS23250 Protein ID WP_004222378.1
Coordinates 4734476..4734742 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMZ10_RS23240 (4732763) 4732763..4733884 + 1122 WP_002887282.1 cupin domain-containing protein -
MMZ10_RS23245 (4733932) 4733932..4734465 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MMZ10_RS23250 (4734476) 4734476..4734742 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MMZ10_RS23255 (4734845) 4734845..4736278 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
MMZ10_RS23260 (4736268) 4736268..4736951 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MMZ10_RS23265 (4737123) 4737123..4738508 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
MMZ10_RS23270 (4738526) 4738526..4738870 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T237711 WP_004178461.1 NZ_CP093151:c4734465-4733932 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T237711 NZ_LR882500:71912-72313 [Mycobacterium tuberculosis variant microti]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT237711 WP_004222378.1 NZ_CP093151:c4734742-4734476 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT237711 NZ_LR882500:71680-71919 [Mycobacterium tuberculosis variant microti]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References