Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 326811..327397 Replicon chromosome
Accession NZ_CP093151
Organism Klebsiella pneumoniae strain CY814036

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MMZ10_RS01525 Protein ID WP_002920800.1
Coordinates 327029..327397 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MMZ10_RS01520 Protein ID WP_004174006.1
Coordinates 326811..327032 (+) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMZ10_RS01500 (322968) 322968..323894 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MMZ10_RS01505 (323891) 323891..325168 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MMZ10_RS01510 (325165) 325165..325932 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MMZ10_RS01515 (325934) 325934..326647 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MMZ10_RS01520 (326811) 326811..327032 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MMZ10_RS01525 (327029) 327029..327397 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MMZ10_RS01530 (327670) 327670..328986 + 1317 WP_032447920.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MMZ10_RS01535 (329093) 329093..329980 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MMZ10_RS01540 (329977) 329977..330822 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MMZ10_RS01545 (330824) 330824..331894 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 321797..332631 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-86)

Antitoxin

(1-50)


Sequences


Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T237698 WP_002920800.1 NZ_CP093151:327029-327397 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T237698 NZ_LR882499:c2971632-2971309 [Mycobacterium tuberculosis variant microti OV254]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCAGTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT237698 WP_004174006.1 NZ_CP093151:326811-327032 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT237698 NZ_LR882499:c2971957-2971712 [Mycobacterium tuberculosis variant microti OV254]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1


Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References