Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 199847..200586 Replicon chromosome
Accession NZ_CP093151
Organism Klebsiella pneumoniae strain CY814036

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag MMZ10_RS00980 Protein ID WP_004901283.1
Coordinates 200101..200586 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MMZ10_RS00975 Protein ID WP_003026799.1
Coordinates 199847..200113 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMZ10_RS00960 (195349) 195349..197418 + 2070 WP_002922127.1 glycine--tRNA ligase subunit beta -
MMZ10_RS00965 (197716) 197716..199085 + 1370 WP_087661561.1 IS3 family transposase -
MMZ10_RS00970 (199286) 199286..199714 + 429 WP_072001701.1 GFA family protein -
MMZ10_RS00975 (199847) 199847..200113 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MMZ10_RS00980 (200101) 200101..200586 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
MMZ10_RS00985 (200930) 200930..201082 + 153 WP_012967075.1 type I toxin-antitoxin system toxin HokA -
MMZ10_RS00990 (201384) 201384..203003 + 1620 WP_040237813.1 ATP-binding cassette domain-containing protein -
MMZ10_RS00995 (203102) 203102..203314 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
MMZ10_RS01000 (203567) 203567..203857 - 291 WP_002921931.1 HTH-type transcriptional regulator -
MMZ10_RS01005 (204103) 204103..205458 - 1356 WP_004188196.1 aromatic acid/H+ symport family MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 198360..199085 725


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T237697 WP_004901283.1 NZ_CP093151:200101-200586 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T237697 NZ_LR882499:2926411-2926851 [Mycobacterium tuberculosis variant microti OV254]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT237697 WP_003026799.1 NZ_CP093151:199847-200113 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT237697 NZ_LR882499:2926137-2926424 [Mycobacterium tuberculosis variant microti OV254]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References