Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 98198..98853 | Replicon | plasmid p104 |
| Accession | NZ_CP093070 | ||
| Organism | Escherichia coli strain BR1220 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | A0A2B7QJJ1 |
| Locus tag | MM431_RS25825 | Protein ID | WP_001441071.1 |
| Coordinates | 98452..98853 (+) | Length | 134 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | A0A0E0YAG4 |
| Locus tag | MM431_RS25820 | Protein ID | WP_001261288.1 |
| Coordinates | 98198..98428 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MM431_RS25780 (93248) | 93248..93589 | + | 342 | Protein_115 | DUF4113 domain-containing protein | - |
| MM431_RS25785 (93621) | 93621..94013 | - | 393 | WP_000340832.1 | plasmid partitioning/stability family protein | - |
| MM431_RS25790 (94018) | 94018..94989 | - | 972 | WP_001103689.1 | plasmid segregation protein ParM | - |
| MM431_RS25795 (95218) | 95218..95862 | + | 645 | WP_000633911.1 | AAA family ATPase | - |
| MM431_RS25800 (95856) | 95856..96131 | + | 276 | WP_000239529.1 | hypothetical protein | - |
| MM431_RS25805 (96269) | 96269..97078 | - | 810 | WP_000016979.1 | site-specific integrase | - |
| MM431_RS25810 (97079) | 97079..97384 | - | 306 | WP_175283076.1 | type II toxin-antitoxin system toxin CcdB | - |
| MM431_RS25815 (97386) | 97386..97604 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| MM431_RS25820 (98198) | 98198..98428 | + | 231 | WP_001261288.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| MM431_RS25825 (98452) | 98452..98853 | + | 402 | WP_001441071.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| MM431_RS25830 (99023) | 99023..101161 | - | 2139 | WP_032269946.1 | ATPase AAA | - |
| MM431_RS25835 (101385) | 101385..102942 | - | 1558 | Protein_126 | IS66-like element ISEc23 family transposase | - |
| MM431_RS25840 (102973) | 102973..103323 | - | 351 | Protein_127 | IS66 family insertion sequence element accessory protein TnpB | - |
| MM431_RS25845 (103320) | 103320..103745 | - | 426 | WP_000422741.1 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | pic / aap/aspU / agg3B / agg3C / agg3D | 1..104757 | 104757 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 134 a.a. Molecular weight: 14517.89 Da Isoelectric Point: 7.2456
>T237695 WP_001441071.1 NZ_CP093070:98452-98853 [Escherichia coli]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFGATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFGATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
Download Length: 402 bp
>T237695 NZ_LR882499:2864898-2865275 [Mycobacterium tuberculosis variant microti OV254]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8684.76 Da Isoelectric Point: 4.3654
>AT237695 WP_001261288.1 NZ_CP093070:98198-98428 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL
Download Length: 231 bp
>AT237695 NZ_LR882499:2864644-2864901 [Mycobacterium tuberculosis variant microti OV254]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2B7QJJ1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0YAG4 |