Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 97079..97604 Replicon plasmid p104
Accession NZ_CP093070
Organism Escherichia coli strain BR1220

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag MM431_RS25810 Protein ID WP_175283076.1
Coordinates 97079..97384 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MM431_RS25815 Protein ID WP_000813634.1
Coordinates 97386..97604 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM431_RS25780 (93248) 93248..93589 + 342 Protein_115 DUF4113 domain-containing protein -
MM431_RS25785 (93621) 93621..94013 - 393 WP_000340832.1 plasmid partitioning/stability family protein -
MM431_RS25790 (94018) 94018..94989 - 972 WP_001103689.1 plasmid segregation protein ParM -
MM431_RS25795 (95218) 95218..95862 + 645 WP_000633911.1 AAA family ATPase -
MM431_RS25800 (95856) 95856..96131 + 276 WP_000239529.1 hypothetical protein -
MM431_RS25805 (96269) 96269..97078 - 810 WP_000016979.1 site-specific integrase -
MM431_RS25810 (97079) 97079..97384 - 306 WP_175283076.1 type II toxin-antitoxin system toxin CcdB Toxin
MM431_RS25815 (97386) 97386..97604 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MM431_RS25820 (98198) 98198..98428 + 231 WP_001261288.1 type II toxin-antitoxin system VapB family antitoxin -
MM431_RS25825 (98452) 98452..98853 + 402 WP_001441071.1 type II toxin-antitoxin system VapC family toxin -
MM431_RS25830 (99023) 99023..101161 - 2139 WP_032269946.1 ATPase AAA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / aap/aspU / agg3B / agg3C / agg3D 1..104757 104757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11682.45 Da        Isoelectric Point: 6.4674

>T237694 WP_175283076.1 NZ_CP093070:c97384-97079 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVSVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T237694 NZ_LR882499:2864192-2864605 [Mycobacterium tuberculosis variant microti OV254]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT237694 WP_000813634.1 NZ_CP093070:c97604-97386 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT237694 NZ_LR882499:2864644-2864901 [Mycobacterium tuberculosis variant microti OV254]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References