Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5208..5767 Replicon plasmid p104
Accession NZ_CP093070
Organism Escherichia coli strain BR1220

Toxin (Protein)


Gene name relE Uniprot ID A0A7B2ZE18
Locus tag MM431_RS25235 Protein ID WP_032250674.1
Coordinates 5208..5483 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag MM431_RS25240 Protein ID WP_001387467.1
Coordinates 5483..5767 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM431_RS25200 (1257) 1257..1997 - 741 WP_001066941.1 tyrosine-type recombinase/integrase -
MM431_RS25205 (2118) 2118..2300 - 183 WP_050488504.1 hypothetical protein -
MM431_RS25210 (2367) 2367..2657 - 291 Protein_3 type II toxin-antitoxin system RelE/ParE family toxin -
MM431_RS25215 (2644) 2644..2912 - 269 Protein_4 type II toxin-antitoxin system ParD family antitoxin -
MM431_RS25220 (3444) 3444..4613 + 1170 WP_032284272.1 hypothetical protein -
MM431_RS25225 (4646) 4646..4774 + 129 Protein_6 IS481 family transposase -
MM431_RS25230 (4899) 4899..5102 - 204 Protein_7 hypothetical protein -
MM431_RS25235 (5208) 5208..5483 - 276 WP_032250674.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MM431_RS25240 (5483) 5483..5767 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
MM431_RS25245 (6681) 6681..7538 - 858 WP_032284166.1 incFII family plasmid replication initiator RepA -
MM431_RS25250 (7531) 7531..7605 - 75 WP_031943482.1 RepA leader peptide Tap -
MM431_RS25260 (7842) 7842..8096 - 255 WP_000083850.1 replication regulatory protein RepA -
MM431_RS25265 (8369) 8369..8503 - 135 WP_023144756.1 Hok/Gef family protein -
- (8556) 8556..8607 + 52 NuclAT_1 - -
- (8556) 8556..8607 + 52 NuclAT_1 - -
- (8556) 8556..8607 + 52 NuclAT_1 - -
- (8556) 8556..8607 + 52 NuclAT_1 - -
MM431_RS25270 (8574) 8574..8860 - 287 Protein_14 DUF2726 domain-containing protein -
MM431_RS25275 (9372) 9372..9584 - 213 WP_001301409.1 ANR family transcriptional regulator -
MM431_RS25280 (9716) 9716..10276 - 561 WP_000139341.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / aap/aspU / agg3B / agg3C / agg3D 1..104757 104757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10750.48 Da        Isoelectric Point: 9.9324

>T237686 WP_032250674.1 NZ_CP093070:c5483-5208 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGELLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T237686 NZ_LR882499:c2233886-2233326 [Mycobacterium tuberculosis variant microti OV254]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT237686 WP_001387467.1 NZ_CP093070:c5767-5483 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT237686 NZ_LR882499:c2234224-2233883 [Mycobacterium tuberculosis variant microti OV254]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7B2ZE18

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References