Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4583568..4584065 Replicon chromosome
Accession NZ_CP093068
Organism Escherichia coli strain BR1220

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag MM431_RS22470 Protein ID WP_038988892.1
Coordinates 4583568..4583741 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MM431_RS22475 Protein ID WP_000398619.1
Coordinates 4583769..4584065 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM431_RS22460 (4580238) 4580238..4581695 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MM431_RS22465 (4581932) 4581932..4583449 + 1518 WP_001295074.1 lysine--tRNA ligase -
MM431_RS22470 (4583568) 4583568..4583741 - 174 WP_038988892.1 type V toxin-antitoxin system toxin GhoT Toxin
MM431_RS22475 (4583769) 4583769..4584065 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MM431_RS22480 (4584292) 4584292..4584564 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
MM431_RS22485 (4584576) 4584576..4584806 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MM431_RS22490 (4584987) 4584987..4586618 + 1632 WP_001216477.1 sensor histidine kinase -
MM431_RS22495 (4586615) 4586615..4587334 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6521.04 Da        Isoelectric Point: 10.2288

>T237685 WP_038988892.1 NZ_CP093068:c4583741-4583568 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSALLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T237685 NZ_LR882499:c2226419-2226000 [Mycobacterium tuberculosis variant microti OV254]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT237685 WP_000398619.1 NZ_CP093068:c4584065-4583769 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT237685 NZ_LR882499:c2226688-2226428 [Mycobacterium tuberculosis variant microti OV254]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References