Detailed information of TA system TA237684

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4374245..4375080
• Replicon: chromosome
• Accession: NZ_CP093068
• Organism: Escherichia coli strain BR1220

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: MM431_RS21395
• Protein ID: WP_077513881.1
• Coordinates: 4374245..4374622 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A403D757
• Locus tag: MM431_RS21400
• Protein ID: WP_016230789.1
• Coordinates: 4374712..4375080 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MM431_RS21365 (4370423)	4370423..4372045	-	1623	WP_241468718.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
MM431_RS21370 (4372278)	4372278..4372505	-	228	WP_024183600.1	hypothetical protein	-
MM431_RS21375 (4372769)	4372769..4372945	-	177	Protein_4206	helix-turn-helix domain-containing protein	-
MM431_RS21380 (4373312)	4373312..4373461	-	150	Protein_4207	hypothetical protein	-
MM431_RS21385 (4373567)	4373567..4373743	-	177	WP_000839286.1	DUF957 domain-containing protein	-
MM431_RS21390 (4373760)	4373760..4374248	-	489	WP_000761690.1	DUF5983 family protein	-
MM431_RS21395 (4374245)	4374245..4374622	-	378	WP_077513881.1	TA system toxin CbtA family protein	Toxin
MM431_RS21400 (4374712)	4374712..4375080	-	369	WP_016230789.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MM431_RS21405 (4375243)	4375243..4375464	-	222	WP_000692345.1	DUF987 domain-containing protein	-
MM431_RS21410 (4375529)	4375529..4376005	-	477	WP_001186774.1	RadC family protein	-
MM431_RS21415 (4376021)	4376021..4376494	-	474	WP_001350782.1	antirestriction protein	-
MM431_RS21420 (4376836)	4376836..4377654	-	819	WP_170884442.1	DUF932 domain-containing protein	-
MM431_RS21425 (4377772)	4377772..4377967	-	196	Protein_4216	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4367488..4393615	26127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14086.22 Da
• Isoelectric Point: 7.7532

>T237684 WP_077513881.1 NZ_CP093068:c4374622-4374245 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T237684 NZ_LR882499:c2215923-2215627 [Mycobacterium tuberculosis variant microti OV254]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13614.45 Da
• Isoelectric Point: 7.0264

>AT237684 WP_016230789.1 NZ_CP093068:c4375080-4374712 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT237684 NZ_LR882499:c2216171-2215920 [Mycobacterium tuberculosis variant microti OV254]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A403D757