Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1003014..1003668 Replicon chromosome
Accession NZ_CP093068
Organism Escherichia coli strain BR1220

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MM431_RS04920 Protein ID WP_000244777.1
Coordinates 1003261..1003668 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MM431_RS04915 Protein ID WP_000354046.1
Coordinates 1003014..1003280 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM431_RS04890 (998183) 998183..998926 + 744 WP_000951964.1 SDR family oxidoreductase -
MM431_RS04895 (998983) 998983..1000416 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
MM431_RS04900 (1000461) 1000461..1000772 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MM431_RS04905 (1000936) 1000936..1001595 + 660 WP_000250274.1 hemolysin III family protein -
MM431_RS04910 (1001791) 1001791..1002771 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MM431_RS04915 (1003014) 1003014..1003280 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MM431_RS04920 (1003261) 1003261..1003668 + 408 WP_000244777.1 protein YgfX Toxin
MM431_RS04925 (1003708) 1003708..1004229 - 522 WP_001055874.1 flavodoxin FldB -
MM431_RS04930 (1004341) 1004341..1005237 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MM431_RS04935 (1005262) 1005262..1005972 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MM431_RS04940 (1005978) 1005978..1007711 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T237664 WP_000244777.1 NZ_CP093068:1003261-1003668 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T237664 NZ_LR882499:719923-720330 [Mycobacterium tuberculosis variant microti OV254]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT237664 WP_000354046.1 NZ_CP093068:1003014-1003280 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT237664 NZ_LR882499:719666-719926 [Mycobacterium tuberculosis variant microti OV254]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References