Detailed information of TA system TA237596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4997876..4998462
• Replicon: chromosome
• Accession: NZ_CP093030
• Organism: Pseudomonas aeruginosa strain H04

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: MMZ67_RS23825
• Protein ID: WP_003120987.1
• Coordinates: 4998163..4998462 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: MMZ67_RS23820
• Protein ID: WP_003448662.1
• Coordinates: 4997876..4998166 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ67_RS23800 (MMZ67_23810)	4993027..4993236	+	210	WP_003105733.1	cold-shock protein	-
MMZ67_RS23805 (MMZ67_23815)	4993458..4995347	+	1890	WP_016851610.1	hypothetical protein	-
MMZ67_RS23810 (MMZ67_23820)	4995344..4997320	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
MMZ67_RS23815 (MMZ67_23825)	4997461..4997805	+	345	WP_016851612.1	hypothetical protein	-
MMZ67_RS23820 (MMZ67_23830)	4997876..4998166	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
MMZ67_RS23825 (MMZ67_23835)	4998163..4998462	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ67_RS23830 (MMZ67_23840)	4998664..4999788	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
MMZ67_RS23835 (MMZ67_23845)	4999788..5001497	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
MMZ67_RS23840 (MMZ67_23850)	5001501..5002826	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
MMZ67_RS23845 (MMZ67_23855)	5002816..5003349	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4970897..5065374	94477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T237596 WP_003120987.1 NZ_CP093030:c4998462-4998163 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T237596 NZ_LR882498:364708-365010 [Mycobacterium tuberculosis variant microti]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT237596 WP_003448662.1 NZ_CP093030:c4998166-4997876 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT237596 NZ_LR882498:364484-364711 [Mycobacterium tuberculosis variant microti]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: G8CP73