Detailed information of TA system TA023758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 2430265..2430910
• Replicon: chromosome
• Accession: NC_012589
• Organism: Sulfolobus islandicus L.S.2.15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C3MLW2
• Locus tag: LS215_RS13205
• Protein ID: WP_012714472.1
• Coordinates: 2430265..2430654 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C3MLW3
• Locus tag: LS215_RS13210
• Protein ID: WP_012714473.1
• Coordinates: 2430641..2430910 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS215_RS13170 (LS215_2643)	2425412..2426179	+	768	WP_012714468.1	formate dehydrogenase accessory sulfurtransferase FdhD	-
LS215_RS13175 (LS215_2644)	2426242..2427180	+	939	WP_012714469.1	PEP/pyruvate-binding domain-containing protein	-
LS215_RS13180 (LS215_2645)	2427245..2428282	-	1038	Protein_2702	ISH3 family transposase	-
LS215_RS13190 (LS215_2646)	2428727..2428957	+	231	WP_012714470.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LS215_RS13195 (LS215_2647)	2428941..2429324	+	384	WP_012714471.1	type II toxin-antitoxin system VapC family toxin	-
LS215_RS13200 (LS215_2648)	2429833..2430054	+	222	WP_048051943.1	hypothetical protein	-
LS215_RS13205 (LS215_2649)	2430265..2430654	-	390	WP_012714472.1	type II toxin-antitoxin system VapC family toxin	Toxin
LS215_RS13210 (LS215_2650)	2430641..2430910	-	270	WP_012714473.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LS215_RS15840 (LS215_2651)	2431068..2431220	+	153	Protein_2708	IS5/IS1182 family transposase	-
LS215_RS15845 (LS215_2652)	2431267..2431638	-	372	Protein_2709	transposase	-
LS215_RS13220 (LS215_2653)	2431656..2432687	-	1032	Protein_2710	ISH3 family transposase	-
LS215_RS13225 (LS215_2654)	2432862..2433149	-	288	WP_012712409.1	ferredoxin family protein	-
LS215_RS13230 (LS215_2655)	2433146..2434333	-	1188	WP_012714475.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2427245..2432516	5271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (4-46)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 15183.64 Da
• Isoelectric Point: 4.7760

>T23758 WP_012714472.1 NC_012589:c2430654-2430265 [Sulfolobus islandicus L.S.2.15]
MIFVDANFIIYLNLGVKEVENFYIKVLQEDRLALDPLVIDEVIYISKKKYNVNFNDTISFLDEIVLPNSLILPIRKEDYD
KAKEIMLQYNLKPSDAFHVAVMLNNSISKIISEDKDFDRTKEIHRVWIT

Nucleotide

• Length: 390 bp

>T23758 NC_012589:c2430654-2430265 [Sulfolobus islandicus L.S.2.15]
ATGATTTTTGTTGACGCAAACTTTATCATATACTTGAATTTGGGTGTAAAGGAAGTAGAAAATTTTTATATTAAAGTCCT
ACAAGAAGATCGACTAGCTTTAGACCCATTAGTTATAGATGAAGTAATTTATATTTCAAAGAAAAAATATAACGTGAACT
TTAACGATACAATAAGTTTTCTAGACGAGATCGTTTTACCTAACTCTCTCATCCTTCCTATAAGAAAAGAAGATTATGAT
AAAGCAAAAGAGATAATGCTACAATATAACCTAAAGCCTTCAGACGCATTTCACGTTGCCGTAATGTTAAATAACTCAAT
TAGTAAAATTATAAGTGAAGATAAGGATTTTGATAGAACTAAAGAAATACATAGAGTTTGGATAACTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10117.82 Da
• Isoelectric Point: 5.7456

>AT23758 WP_012714473.1 NC_012589:c2430910-2430641 [Sulfolobus islandicus L.S.2.15]
MTLKVEVGKKGYIIIPKDIRDLLGIKEGDKLILNVYDGKIILEPERKVDINEILKRLEGHHAKISAYAKSAKLGDLLYSS
LEEEFDDFC

Nucleotide

• Length: 270 bp

>AT23758 NC_012589:c2430910-2430641 [Sulfolobus islandicus L.S.2.15]
TTGACTCTAAAAGTGGAAGTTGGAAAGAAAGGTTACATAATTATTCCTAAGGATATCAGAGATCTCCTAGGCATAAAAGA
AGGTGACAAGCTTATTCTTAACGTCTATGACGGTAAAATTATCCTTGAACCTGAAAGGAAGGTTGACATTAATGAGATCT
TAAAAAGACTTGAGGGACATCACGCTAAAATATCCGCTTATGCAAAAAGCGCTAAATTAGGAGACTTGCTTTATTCGAGC
CTTGAGGAGGAATTTGATGATTTTTGTTGA

Structures

Toxin

• AlphaFold DB: C3MLW2

Antitoxin

• AlphaFold DB: C3MLW3