Detailed information of TA system TA237570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2482372..2483414
• Replicon: chromosome
• Accession: NZ_CP093022
• Organism: Pseudomonas aeruginosa strain H08

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: MMZ71_RS11875
• Protein ID: WP_003109777.1
• Coordinates: 2482839..2483414 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: MMZ71_RS11870
• Protein ID: WP_003050245.1
• Coordinates: 2482372..2482842 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ71_RS11835 (MMZ71_11840)	2477764..2479182	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
MMZ71_RS11840 (MMZ71_11845)	2479172..2480083	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
MMZ71_RS11845 (MMZ71_11850)	2480080..2480772	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
MMZ71_RS11850 (MMZ71_11855)	2480769..2481167	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
MMZ71_RS11855 (MMZ71_11860)	2481179..2481538	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
MMZ71_RS11860 (MMZ71_11865)	2481555..2481788	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
MMZ71_RS11865 (MMZ71_11870)	2481785..2482168	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
MMZ71_RS11870 (MMZ71_11875)	2482372..2482842	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
MMZ71_RS11875 (MMZ71_11880)	2482839..2483414	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
MMZ71_RS11880 (MMZ71_11885)	2483432..2484346	+	915	WP_003105629.1	AAA family ATPase	-
MMZ71_RS11885 (MMZ71_11890)	2484343..2484813	+	471	WP_003105626.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
MMZ71_RS11890 (MMZ71_11895)	2484810..2485310	+	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
MMZ71_RS11895 (MMZ71_11900)	2485310..2486212	+	903	WP_003105624.1	CBASS oligonucleotide cyclase	-
MMZ71_RS11900 (MMZ71_11905)	2486251..2486976	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2432534..2528818	96284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T237570 WP_003109777.1 NZ_CP093022:2482839-2483414 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T237570 NZ_LR882497:c2234139-2233579 [Mycobacterium tuberculosis variant microti]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT237570 WP_003050245.1 NZ_CP093022:2482372-2482842 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT237570 NZ_LR882497:c2234477-2234136 [Mycobacterium tuberculosis variant microti]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Structures

Antitoxin

• AlphaFold DB: I3TV68