Detailed information of TA system TA023757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/NGN_SP-AbrB
• Location: 2428727..2429324
• Replicon: chromosome
• Accession: NC_012589
• Organism: Sulfolobus islandicus L.S.2.15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D2PH32
• Locus tag: LS215_RS13195
• Protein ID: WP_012714471.1
• Coordinates: 2428941..2429324 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D2PH31
• Locus tag: LS215_RS13190
• Protein ID: WP_012714470.1
• Coordinates: 2428727..2428957 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS215_RS13165 (LS215_2642)	2425075..2425431	+	357	WP_012712406.1	DUF1641 domain-containing protein	-
LS215_RS13170 (LS215_2643)	2425412..2426179	+	768	WP_012714468.1	formate dehydrogenase accessory sulfurtransferase FdhD	-
LS215_RS13175 (LS215_2644)	2426242..2427180	+	939	WP_012714469.1	PEP/pyruvate-binding domain-containing protein	-
LS215_RS13180 (LS215_2645)	2427245..2428282	-	1038	Protein_2702	ISH3 family transposase	-
LS215_RS13190 (LS215_2646)	2428727..2428957	+	231	WP_012714470.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LS215_RS13195 (LS215_2647)	2428941..2429324	+	384	WP_012714471.1	type II toxin-antitoxin system VapC family toxin	Toxin
LS215_RS13200 (LS215_2648)	2429833..2430054	+	222	WP_048051943.1	hypothetical protein	-
LS215_RS13205 (LS215_2649)	2430265..2430654	-	390	WP_012714472.1	type II toxin-antitoxin system VapC family toxin	-
LS215_RS13210 (LS215_2650)	2430641..2430910	-	270	WP_012714473.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LS215_RS15840 (LS215_2651)	2431068..2431220	+	153	Protein_2708	IS5/IS1182 family transposase	-
LS215_RS15845 (LS215_2652)	2431267..2431638	-	372	Protein_2709	transposase	-
LS215_RS13220 (LS215_2653)	2431656..2432687	-	1032	Protein_2710	ISH3 family transposase	-
LS215_RS13225 (LS215_2654)	2432862..2433149	-	288	WP_012712409.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2427245..2432516	5271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-118)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14334.73 Da
• Isoelectric Point: 9.4868

>T23757 WP_012714471.1 NC_012589:2428941-2429324 [Sulfolobus islandicus L.S.2.15]
VKVVLDASTLILYFYGNKKAKEIIEKNMNEAFVNSVNLTEFLYSYAKVKGWKESLLKYSLIRNAFELIDVNDEDIIKSTA
KLKIKYNLSLGDAFLLASAKSINSTAVTSDHELSKVKEVKVILIPKE

Nucleotide

• Length: 384 bp

>T23757 NC_012589:2428941-2429324 [Sulfolobus islandicus L.S.2.15]
GTGAAAGTAGTACTTGACGCCTCTACATTAATTTTGTATTTTTATGGAAATAAAAAAGCAAAAGAGATAATAGAGAAAAA
TATGAATGAGGCTTTTGTAAATTCAGTAAATCTTACAGAATTCCTTTATTCATACGCAAAAGTCAAAGGTTGGAAAGAAT
CCCTATTAAAATATTCTTTAATAAGAAACGCCTTTGAGCTAATAGATGTTAACGATGAGGATATAATTAAAAGCACAGCT
AAGTTGAAAATTAAGTATAACCTCTCCTTAGGAGATGCTTTTTTATTAGCATCTGCTAAAAGTATAAATTCAACAGCTGT
AACGAGCGATCACGAGCTTAGTAAAGTTAAAGAGGTTAAGGTAATACTGATTCCTAAGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9053.35 Da
• Isoelectric Point: 5.9093

>AT23757 WP_012714470.1 NC_012589:2428727-2428957 [Sulfolobus islandicus L.S.2.15]
MYRLKVHKKGIIVIPKEIREKFNLKEGDEVNVIVDDDGIYIFPRDDIRKYFGSDKDAIKALDILEEERRRERESST

Nucleotide

• Length: 231 bp

>AT23757 NC_012589:2428727-2428957 [Sulfolobus islandicus L.S.2.15]
ATGTATAGATTGAAGGTGCATAAGAAAGGTATTATTGTAATACCGAAGGAGATAAGAGAAAAATTCAACTTGAAAGAAGG
TGACGAAGTTAACGTTATAGTTGACGATGATGGAATTTATATATTTCCTCGTGATGATATTAGAAAATACTTTGGTAGTG
ACAAGGACGCAATAAAAGCTTTAGACATTCTGGAAGAGGAGAGGAGAAGAGAACGTGAAAGTAGTACTTGA

Structures

Toxin

• AlphaFold DB: D2PH32

Antitoxin

• AlphaFold DB: D2PH31