Detailed information of TA system TA023756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 2223813..2224419
• Replicon: chromosome
• Accession: NC_012589
• Organism: Sulfolobus islandicus L.S.2.15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q97UE3
• Locus tag: LS215_RS12225
• Protein ID: WP_009989090.1
• Coordinates: 2224015..2224419 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9UBQ9
• Locus tag: LS215_RS12220
• Protein ID: WP_009989091.1
• Coordinates: 2223813..2224034 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS215_RS12190 (LS215_2446)	2218820..2219437	+	618	WP_012712231.1	hypothetical protein	-
LS215_RS12195 (LS215_2447)	2219434..2219820	+	387	WP_012714323.1	hypothetical protein	-
LS215_RS12200 (LS215_2448)	2219817..2220482	-	666	WP_012714324.1	helix-turn-helix domain-containing protein	-
LS215_RS12205 (LS215_2449)	2220661..2221363	+	703	Protein_2499	IS6 family transposase	-
LS215_RS12210 (LS215_2451)	2221417..2222688	-	1272	WP_012714325.1	MFS transporter	-
LS215_RS12215 (LS215_2452)	2222799..2223029	-	231	WP_052293595.1	multidrug DMT transporter permease	-
LS215_RS15800	2223083..2223343	-	261	WP_167525052.1	hypothetical protein	-
LS215_RS12220 (LS215_2454)	2223813..2224034	+	222	WP_009989091.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LS215_RS12225 (LS215_2455)	2224015..2224419	+	405	WP_009989090.1	PIN domain-containing protein	Toxin
LS215_RS15805	2224617..2224904	-	288	WP_274378383.1	transposase	-
LS215_RS16095	2224946..2225053	+	108	Protein_2506	IS110 family transposase	-
LS215_RS12235	2225158..2225776	-	619	Protein_2507	transposase	-
LS215_RS12240 (LS215_2458)	2226217..2226996	-	780	WP_014511459.1	DMT family transporter	-
LS215_RS12245	2227832..2228014	+	183	WP_012953209.1	hypothetical protein	-
LS215_RS12250 (LS215_2460)	2228162..2228923	+	762	WP_012714327.1	ZIP family metal transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2220661..2225776	5115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (4-48)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15426.98 Da
• Isoelectric Point: 5.0272

>T23756 WP_009989090.1 NC_012589:2224015-2224419 [Sulfolobus islandicus L.S.2.15]
VEKAIVDTNVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMWFLKGMKLEDKVDDVLAYIRSEKAEVVCDCTDNV
TDAIGVLNKEKLPLVSYKDMVILSHAIREKLPLATFDRKLSKIAKKYSVSLLQS

Nucleotide

• Length: 405 bp

>T23756 NC_012589:2224015-2224419 [Sulfolobus islandicus L.S.2.15]
GTGGAGAAGGCAATAGTTGACACTAACGTGATAATTTACGACTACGTTGAAGACTCCGAATATCATAAGAGGGCGGAAGA
GCTTCTCGATAGTTTGGATAGGTGGGTAATTCCCGTGATTGTAGTTCATGAGATCATGTGGTTCTTAAAGGGCATGAAAC
TTGAAGACAAGGTAGATGACGTTTTGGCTTATATTAGAAGTGAGAAAGCTGAGGTTGTCTGCGATTGTACAGATAACGTC
ACTGACGCTATAGGAGTGCTGAATAAGGAAAAGCTTCCCTTGGTCAGCTACAAAGATATGGTAATACTTTCTCACGCAAT
TAGAGAAAAATTGCCTTTAGCCACTTTTGATAGGAAACTCTCAAAGATAGCAAAGAAGTACTCCGTAAGTTTGCTACAGT
CGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8267.50 Da
• Isoelectric Point: 9.6908

>AT23756 WP_009989091.1 NC_012589:2223813-2224034 [Sulfolobus islandicus L.S.2.15]
MERVKVTRNYQITIPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGSKLSLGDIEEAIERGEGNS

Nucleotide

• Length: 222 bp

>AT23756 NC_012589:2223813-2224034 [Sulfolobus islandicus L.S.2.15]
ATGGAGAGGGTAAAGGTTACGAGAAATTACCAGATAACTATTCCAGCCTCGGCTAGGAGTAAGATTAATATAAAAGAAGG
AGATATACTTGAGGTTTACTTGAACGGTGACGAGATAGTACTTCGGAAGGTTAAGACTGAGAGGCCGAGGATAAGGCTAG
GCAGTAAACTCTCACTGGGAGATATTGAGGAGGCAATAGAGCGTGGAGAAGGCAATAGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E3K068

Antitoxin

• AlphaFold DB: M9UBQ9