Detailed information of TA system TA237554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 178334..179007
• Replicon: plasmid unnamed
• Accession: NZ_CP093017
• Organism: Pseudomonas aeruginosa strain H15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MMZ75_RS32775
• Protein ID: WP_241474555.1
• Coordinates: 178564..179007 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MMZ75_RS32770
• Protein ID: WP_096265180.1
• Coordinates: 178334..178564 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ75_RS32740 (MMZ75_32750)	173493..173846	-	354	WP_042920904.1	hypothetical protein	-
MMZ75_RS32745 (MMZ75_32755)	173897..174973	-	1077	WP_241474552.1	hypothetical protein	-
MMZ75_RS32750 (MMZ75_32760)	175124..176167	-	1044	WP_241474553.1	hypothetical protein	-
MMZ75_RS32755 (MMZ75_32765)	176188..176655	-	468	WP_241474554.1	hypothetical protein	-
MMZ75_RS32760 (MMZ75_32770)	176655..176888	-	234	WP_042920911.1	hypothetical protein	-
MMZ75_RS32765 (MMZ75_32775)	177455..177817	-	363	WP_045632674.1	DNA binding protein	-
MMZ75_RS32770 (MMZ75_32780)	178334..178564	+	231	WP_096265180.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MMZ75_RS32775 (MMZ75_32785)	178564..179007	+	444	WP_241474555.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MMZ75_RS32780 (MMZ75_32790)	179046..179504	+	459	WP_162962050.1	hypothetical protein	-
MMZ75_RS32785 (MMZ75_32795)	179531..179881	+	351	WP_162962051.1	hypothetical protein	-
MMZ75_RS32790 (MMZ75_32800)	180222..180803	+	582	WP_241474556.1	hypothetical protein	-
MMZ75_RS32795 (MMZ75_32805)	180822..181103	+	282	WP_121498204.1	hypothetical protein	-
MMZ75_RS32800 (MMZ75_32810)	181114..181632	+	519	WP_241474557.1	hypothetical protein	-
MMZ75_RS32805 (MMZ75_32815)	181811..182035	+	225	WP_121498206.1	hypothetical protein	-
MMZ75_RS32810 (MMZ75_32820)	182321..182488	+	168	WP_162962052.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..436805	436805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16423.77 Da
• Isoelectric Point: 6.8646

>T237554 WP_241474555.1 NZ_CP093017:178564-179007 [Pseudomonas aeruginosa]
MLKYMLDTNICIFTIKNRPQEVREAFKRHHGQMCISTVTLMELIYGAEKSSNPERNLADVEGFAARLEVLKYDQGAAEHT
GQLRAELARLGQPIGPYDQMVAGHARSQGLIVVTNNRREFERVPGLRVEDWVGKEPGSTAETGALLV

Nucleotide

• Length: 444 bp

>T237554 NZ_LR882497:1016332-1016766 [Mycobacterium tuberculosis variant microti]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8433.33 Da
• Isoelectric Point: 4.3719

>AT237554 WP_096265180.1 NZ_CP093017:178334-178564 [Pseudomonas aeruginosa]
VEQGAVFQSNRSQAIRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDSNGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT237554 NZ_LR882497:1016147-1016326 [Mycobacterium tuberculosis variant microti]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG