Detailed information of TA system TA237544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5401867..5402507
• Replicon: chromosome
• Accession: NZ_CP093015
• Organism: Pseudomonas aeruginosa strain H16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MMZ76_RS25210
• Protein ID: WP_003105740.1
• Coordinates: 5401867..5402277 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6N0KGL5
• Locus tag: MMZ76_RS25215
• Protein ID: WP_023132185.1
• Coordinates: 5402277..5402507 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ76_RS25165 (MMZ76_25170)	5397023..5398015	-	993	WP_241476894.1	hypothetical protein	-
MMZ76_RS25170 (MMZ76_25175)	5398092..5398820	+	729	WP_094954010.1	TIGR03761 family integrating conjugative element protein	-
MMZ76_RS25175 (MMZ76_25180)	5398826..5399374	+	549	WP_094954011.1	DUF3158 family protein	-
MMZ76_RS25180 (MMZ76_25185)	5399422..5400252	+	831	WP_094954012.1	Rha family transcriptional regulator	-
MMZ76_RS25185 (MMZ76_25190)	5400282..5400770	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
MMZ76_RS25190 (MMZ76_25195)	5400934..5401101	+	168	WP_128729268.1	CrpP family ICE-associated protein	-
MMZ76_RS25195 (MMZ76_25200)	5401167..5401385	-	219	WP_003105747.1	hypothetical protein	-
MMZ76_RS25200 (MMZ76_25205)	5401399..5401581	-	183	WP_009315855.1	hypothetical protein	-
MMZ76_RS25205 (MMZ76_25210)	5401585..5401845	-	261	WP_003105742.1	hypothetical protein	-
MMZ76_RS25210 (MMZ76_25215)	5401867..5402277	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MMZ76_RS25215 (MMZ76_25220)	5402277..5402507	-	231	WP_023132185.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MMZ76_RS25220 (MMZ76_25225)	5402826..5404745	+	1920	WP_031627730.1	type I DNA topoisomerase	-
MMZ76_RS25225 (MMZ76_25230)	5404753..5405505	+	753	WP_009315847.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5382499..5486937	104438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T237544 WP_003105740.1 NZ_CP093015:c5402277-5401867 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T237544 NZ_LR882497:c699039-698626 [Mycobacterium tuberculosis variant microti]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8468.35 Da
• Isoelectric Point: 4.2821

>AT237544 WP_023132185.1 NZ_CP093015:c5402507-5402277 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTSDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT237544 NZ_LR882497:c699272-699036 [Mycobacterium tuberculosis variant microti]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6N0KGL5