Detailed information of TA system TA237543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5012230..5012838
• Replicon: chromosome
• Accession: NZ_CP093015
• Organism: Pseudomonas aeruginosa strain H16

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: MMZ76_RS23390
• Protein ID: WP_034024253.1
• Coordinates: 5012230..5012577 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: MMZ76_RS23395
• Protein ID: WP_003114155.1
• Coordinates: 5012587..5012838 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ76_RS23360 (MMZ76_23365)	5007589..5007861	+	273	WP_073656953.1	cysteine-rich CWC family protein	-
MMZ76_RS23365 (MMZ76_23370)	5007861..5008553	+	693	WP_003085458.1	16S rRNA pseudouridine(516) synthase	-
MMZ76_RS23370 (MMZ76_23375)	5008689..5009732	+	1044	WP_058150052.1	L,D-transpeptidase	-
MMZ76_RS23375 (MMZ76_23380)	5009812..5010549	+	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
MMZ76_RS23380 (MMZ76_23385)	5011001..5011903	+	903	WP_003085447.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
MMZ76_RS23390 (MMZ76_23395)	5012230..5012577	-	348	WP_034024253.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ76_RS23395 (MMZ76_23400)	5012587..5012838	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MMZ76_RS23400 (MMZ76_23405)	5013052..5014035	-	984	WP_033942044.1	tyrosine-type recombinase/integrase	-
MMZ76_RS23405 (MMZ76_23410)	5014035..5015327	-	1293	WP_094953959.1	hypothetical protein	-
MMZ76_RS23410 (MMZ76_23415)	5015557..5016840	-	1284	WP_023124144.1	zonular occludens toxin family protein	-
MMZ76_RS23415 (MMZ76_23420)	5016844..5017200	-	357	WP_003114150.1	DUF2523 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	5012230..5035101	22871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12972.72 Da
• Isoelectric Point: 4.4212

>T237543 WP_034024253.1 NZ_CP093015:c5012577-5012230 [Pseudomonas aeruginosa]
MSPVVIRYTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T237543 NZ_LR882497:c696703-696311 [Mycobacterium tuberculosis variant microti]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT237543 WP_003114155.1 NZ_CP093015:c5012838-5012587 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT237543 NZ_LR882497:c696957-696700 [Mycobacterium tuberculosis variant microti]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355