Detailed information of TA system TA023754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 668346..668952
• Replicon: chromosome
• Accession: NC_012589
• Organism: Sulfolobus islandicus L.S.2.15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C3MMX8
• Locus tag: LS215_RS03700
• Protein ID: WP_012713213.1
• Coordinates: 668346..668738 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C3MME7
• Locus tag: LS215_RS03705
• Protein ID: WP_012713168.1
• Coordinates: 668728..668952 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS215_RS03690 (LS215_0754)	664519..665283	+	765	WP_012713211.1	enoyl-CoA hydratase/isomerase family protein	-
LS215_RS03695 (LS215_0755)	665286..667553	+	2268	WP_012713212.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LS215_RS15645 (LS215_0756)	667630..667830	-	201	Protein_768	hypothetical protein	-
LS215_RS15650	667802..667915	+	114	Protein_769	IS5/IS1182 family transposase	-
LS215_RS03700 (LS215_0757)	668346..668738	-	393	WP_012713213.1	type II toxin-antitoxin system VapC family toxin	Toxin
LS215_RS03705 (LS215_0758)	668728..668952	-	225	WP_012713168.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LS215_RS16415	669088..669266	-	179	Protein_772	CRISPR-associated endoribonuclease Cas6	-
LS215_RS14825 (LS215_0760)	669654..670022	-	369	WP_012713215.1	hypothetical protein	-
LS215_RS14830 (LS215_3031)	670151..670501	-	351	WP_012713216.1	ATP-dependent helicase	-
LS215_RS14835 (LS215_2995)	670504..671406	-	903	WP_012713217.1	hypothetical protein	-
LS215_RS16420 (LS215_0761)	671481..672317	+	837	WP_012713218.1	AMP-binding protein	-
LS215_RS16425 (LS215_2996)	672369..672698	+	330	WP_012713219.1	AMP-dependent ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (9-124)

Antitoxin

• Post-segregation antitoxin CcdA (5-44)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15121.86 Da
• Isoelectric Point: 8.2957

>T23754 WP_012713213.1 NC_012589:c668738-668346 [Sulfolobus islandicus L.S.2.15]
MKDKEFLFDASALYSLLDYVDKIDLKKIHILTLTFYEVGNVIWKEYYIHKKVKDPITLSMLFHKLMRKLNVVEDPPLEGV
MRIAVERGLTYYDASYAYVAESLGLILVSNDKELIRKTNAISLKDLIKSM

Nucleotide

• Length: 393 bp

>T23754 NC_012589:c668738-668346 [Sulfolobus islandicus L.S.2.15]
ATGAAAGATAAGGAATTCCTATTCGATGCCTCAGCTTTGTACTCGCTTTTAGATTACGTGGATAAAATAGACTTAAAGAA
AATTCATATACTTACCCTAACTTTTTATGAGGTAGGTAACGTCATATGGAAGGAGTATTATATACATAAAAAGGTTAAAG
ATCCTATAACTCTTTCGATGCTTTTCCATAAATTAATGCGAAAACTTAACGTAGTAGAGGATCCACCTCTTGAGGGAGTA
ATGAGAATTGCCGTAGAAAGGGGTTTGACTTACTATGACGCATCTTATGCATATGTTGCTGAATCTTTAGGACTTATCCT
AGTGTCTAACGATAAAGAGCTGATAAGAAAAACTAATGCCATTTCGCTAAAGGATTTGATAAAAAGTATGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8880.30 Da
• Isoelectric Point: 6.4451

>AT23754 WP_012713168.1 NC_012589:c668952-668728 [Sulfolobus islandicus L.S.2.15]
MSDVISVRVKKELKKRAEELGINIREVVEKALKEAIREKEKEELKDIVMRIKELMRDVSEDDWVRAVRESRDER

Nucleotide

• Length: 225 bp

>AT23754 NC_012589:c668952-668728 [Sulfolobus islandicus L.S.2.15]
ATGTCTGACGTAATAAGCGTAAGAGTTAAGAAAGAGCTGAAGAAGAGGGCAGAGGAGTTGGGGATTAACATTAGGGAAGT
TGTAGAAAAAGCTTTAAAGGAAGCCATAAGGGAGAAGGAGAAGGAAGAGCTTAAGGATATAGTTATGAGAATTAAGGAAC
TAATGAGGGATGTAAGTGAAGATGATTGGGTAAGGGCTGTTAGGGAGAGTAGGGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: C3MMX8

Antitoxin

• AlphaFold DB: C3MME7