Detailed information of TA system TA023753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 598107..598713
• Replicon: chromosome
• Accession: NC_012589
• Organism: Sulfolobus islandicus L.S.2.15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C3MME8
• Locus tag: LS215_RS03390
• Protein ID: WP_012713169.1
• Coordinates: 598321..598713 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C3MME7
• Locus tag: LS215_RS03385
• Protein ID: WP_012713168.1
• Coordinates: 598107..598331 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS215_RS03365 (LS215_0690)	594622..595787	-	1166	Protein_703	divalent metal cation transporter	-
LS215_RS03370 (LS215_0691)	596096..596677	+	582	WP_012713165.1	DNA protection during starvation protein	-
LS215_RS03375 (LS215_0692)	596708..597046	+	339	WP_012713166.1	Rieske 2Fe-2S domain-containing protein	-
LS215_RS03380 (LS215_0693)	597072..597608	-	537	WP_012713167.1	CRISPR-associated ring nuclease Crn1	-
LS215_RS03385 (LS215_0694)	598107..598331	+	225	WP_012713168.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LS215_RS03390 (LS215_0695)	598321..598713	+	393	WP_012713169.1	type II toxin-antitoxin system VapC family toxin	Toxin
LS215_RS16395	599291..599483	-	193	Protein_709	hypothetical protein	-
LS215_RS03395 (LS215_0696)	599648..599917	+	270	Protein_710	transposase	-
LS215_RS03400 (LS215_0697)	599999..600391	-	393	Protein_711	transposase	-
LS215_RS16400	600661..600849	-	189	WP_238526076.1	hypothetical protein	-
LS215_RS14815 (LS215_0699)	600977..601615	-	639	WP_012713172.1	CRISPR system precrRNA processing endoribonuclease RAMP protein Cas6	-
LS215_RS03415 (LS215_0700)	601694..602335	-	642	WP_012713173.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (9-124)

Antitoxin

• Post-segregation antitoxin CcdA (5-44)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15211.97 Da
• Isoelectric Point: 8.2957

>T23753 WP_012713169.1 NC_012589:598321-598713 [Sulfolobus islandicus L.S.2.15]
MKDKEFLFDASALYSLLDYVDKIDLKKIHILTLTFYEVGNVIWKEYYIHKKVKDPITLSMLFHKLMRKLNVVEDPPLEGV
MRIAVERGLTYYDASYAYVAESLGLILVSNDKELIRKTNAISLKEFIKFI

Nucleotide

• Length: 393 bp

>T23753 NC_012589:598321-598713 [Sulfolobus islandicus L.S.2.15]
ATGAAAGATAAGGAATTCCTATTCGATGCCTCAGCTTTGTACTCGCTTTTAGATTACGTGGATAAAATAGACTTAAAGAA
AATTCATATACTTACCCTAACTTTTTATGAGGTAGGTAACGTCATATGGAAGGAGTATTATATACATAAAAAGGTTAAAG
ATCCTATAACTCTTTCGATGCTTTTCCATAAATTAATGCGAAAACTTAACGTAGTAGAGGATCCACCTCTTGAGGGAGTA
ATGAGAATTGCCGTAGAAAGGGGTTTGACTTACTATGACGCATCTTATGCATATGTTGCTGAATCTTTAGGACTTATCCT
AGTGTCTAACGATAAAGAGCTGATAAGAAAAACTAATGCCATTTCGCTAAAGGAGTTCATTAAATTTATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8880.30 Da
• Isoelectric Point: 6.4451

>AT23753 WP_012713168.1 NC_012589:598107-598331 [Sulfolobus islandicus L.S.2.15]
MSDVISVRVKKELKKRAEELGINIREVVEKALKEAIREKEKEELKDIVMRIKELMRDVSEDDWVRAVRESRDER

Nucleotide

• Length: 225 bp

>AT23753 NC_012589:598107-598331 [Sulfolobus islandicus L.S.2.15]
ATGTCTGACGTAATAAGCGTAAGAGTTAAGAAAGAGCTGAAGAAGAGGGCAGAGGAGTTGGGGATTAACATTAGGGAAGT
TGTAGAAAAAGCTTTAAAGGAAGCCATAAGGGAGAAGGAGAAGGAAGAGCTTAAGGATATAGTTATGAGAATTAAGGAAC
TAATGAGGGATGTAAGTGAAGATGATTGGGTAAGGGCTGTTAGGGAGAGTAGGGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: C3MME8

Antitoxin

• AlphaFold DB: C3MME7