Detailed information of TA system TA023752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 2090011..2090590
• Replicon: chromosome
• Accession: NC_012588
• Organism: Sulfolobus islandicus M.14.25

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C3MS76
• Locus tag: M1425_RS11440
• Protein ID: WP_012712237.1
• Coordinates: 2090186..2090590 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C4KKM5
• Locus tag: M1425_RS11435
• Protein ID: WP_012712236.1
• Coordinates: 2090011..2090205 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1425_RS11405 (M1425_2278)	2085360..2086235	+	876	WP_012712230.1	ABC transporter ATP-binding protein	-
M1425_RS11410 (M1425_2279)	2086226..2086843	+	618	WP_012712231.1	hypothetical protein	-
M1425_RS11415 (M1425_2280)	2086840..2087226	+	387	WP_009989099.1	hypothetical protein	-
M1425_RS11420 (M1425_2281)	2087223..2087933	-	711	WP_012712232.1	helix-turn-helix domain-containing protein	-
M1425_RS11425 (M1425_2282)	2088007..2089278	-	1272	WP_012712233.1	MFS transporter	-
M1425_RS11430 (M1425_2283)	2089333..2089620	-	288	WP_012736143.1	EamA family transporter	-
M1425_RS15220 (M1425_2284)	2089723..2089935	-	213	WP_049780006.1	hypothetical protein	-
M1425_RS11435 (M1425_2285)	2090011..2090205	+	195	WP_012712236.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
M1425_RS11440 (M1425_2286)	2090186..2090590	+	405	WP_012712237.1	PIN domain-containing protein	Toxin
M1425_RS14630 (M1425_2287)	2090786..2091703	-	918	Protein_2315	IS110 family transposase	-
M1425_RS11455 (M1425_2288)	2092152..2092931	-	780	WP_016732287.1	DMT family transporter	-
M1425_RS11460	2093767..2093949	+	183	WP_009990866.1	hypothetical protein	-
M1425_RS11465 (M1425_2290)	2094097..2094858	+	762	WP_009990867.1	ZIP family metal transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (6-39)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15299.87 Da
• Isoelectric Point: 5.0349

>T23752 WP_012712237.1 NC_012588:2090186-2090590 [Sulfolobus islandicus M.14.25]
VEKAIVDTSVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMSFLKGMKLEDKVDDVLAYSRSEKAEVVCDCTDNV
IDAIGVLKKEKLPLISYKNMVILSHAIREKLPLATFDRELSKIAKKYSVSLLLS

Nucleotide

• Length: 405 bp

>T23752 NC_012588:2090186-2090590 [Sulfolobus islandicus M.14.25]
GTGGAGAAGGCAATAGTTGACACTAGCGTGATAATTTACGACTACGTTGAAGACTCCGAATATCATAAGAGGGCTGAAGA
GCTTCTCGATAGTTTGGATAGGTGGGTAATTCCCGTGATTGTAGTTCATGAGATCATGTCGTTCTTAAAGGGCATGAAAC
TTGAAGACAAGGTAGATGACGTTTTGGCTTATAGTAGAAGTGAGAAAGCTGAGGTTGTGTGCGATTGTACAGATAACGTC
ATTGACGCTATAGGAGTGCTGAAGAAGGAAAAGCTTCCCTTGATCAGCTACAAAAATATGGTAATACTTTCTCACGCAAT
TAGAGAAAAATTGCCTTTAGCCACTTTTGATAGGGAACTCTCAAAGATAGCAAAGAAGTACTCCGTAAGTTTGCTACTGT
CGTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7122.17 Da
• Isoelectric Point: 8.6155

>AT23752 WP_012712236.1 NC_012588:2090011-2090205 [Sulfolobus islandicus M.14.25]
MIKSTPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGNKLSLGDIEEAIERGEGNS

Nucleotide

• Length: 195 bp

>AT23752 NC_012588:2090011-2090205 [Sulfolobus islandicus M.14.25]
TTGATTAAGAGCACTCCAGCCTCGGCTAGGAGTAAGATTAATATAAAAGAAGGAGATATACTTGAGGTTTACTTGAACGG
TGACGAGATAGTACTTCGGAAGGTTAAGACTGAGAGGCCGAGGATAAGGCTAGGCAATAAACTCTCACTGGGAGATATTG
AGGAGGCAATAGAGCGTGGAGAAGGCAATAGTTGA

Structures

Toxin

• AlphaFold DB: C3MS76

Antitoxin

• AlphaFold DB: C4KKM5