Detailed information of TA system TA023751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 894479..895082
• Replicon: chromosome
• Accession: NC_012588
• Organism: Sulfolobus islandicus M.14.25

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C4KG62
• Locus tag: M1425_RS04635
• Protein ID: WP_012710993.1
• Coordinates: 894479..894868 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9UC56
• Locus tag: M1425_RS04640
• Protein ID: WP_012710994.1
• Coordinates: 894855..895082 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1425_RS04615 (M1425_0930)	890946..891323	-	378	WP_228375605.1	hypothetical protein	-
M1425_RS14330 (M1425_0932)	892029..893177	+	1149	Protein_947	RNA-guided endonuclease TnpB family protein	-
M1425_RS04630 (M1425_0934)	893520..893690	-	171	Protein_948	IS110 family transposase	-
M1425_RS14825 (M1425_0936)	893977..894081	+	105	Protein_949	IS5/IS1182 family transposase	-
M1425_RS04635 (M1425_0937)	894479..894868	-	390	WP_012710993.1	PIN domain-containing protein	Toxin
M1425_RS04640 (M1425_0938)	894855..895082	-	228	WP_012710994.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
M1425_RS04645 (M1425_0939)	895463..896471	-	1009	Protein_952	ISH3 family transposase	-
M1425_RS04650 (M1425_0940)	896617..896934	-	318	WP_012710995.1	AAA family ATPase	-
M1425_RS04655 (M1425_0941)	897341..897586	+	246	WP_012710996.1	hypothetical protein	-
M1425_RS04660 (M1425_0942)	897583..897993	+	411	WP_012710997.1	PIN domain-containing protein	-
M1425_RS04665 (M1425_0943)	898120..898338	+	219	Protein_956	IS1 family transposase	-
M1425_RS04670 (M1425_0944)	898404..899141	+	738	WP_012710998.1	CorA family divalent cation transporter	-
M1425_RS04675 (M1425_0945)	899273..899803	-	531	WP_012710999.1	dTDP-4-dehydrorhamnose 3,5-epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (3-47)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14829.41 Da
• Isoelectric Point: 4.7430

>T23751 WP_012710993.1 NC_012588:c894868-894479 [Sulfolobus islandicus M.14.25]
MIVVDTNILVYSTFEDSENHSKALEIIEKEDVKIPQIVVYEFLWVLAKLTSDVSLIKTKIEELREFEIICENPETILNGV
KMLKEDGKPLKMLNDYVILALAKELKGDLATYDEKLRKIAEKHGVRIIP

Nucleotide

• Length: 390 bp

>T23751 NC_012588:c894868-894479 [Sulfolobus islandicus M.14.25]
ATGATAGTAGTTGACACGAACATATTAGTTTATTCAACTTTTGAAGATTCCGAAAACCATTCTAAAGCTCTAGAAATTAT
AGAAAAGGAGGACGTTAAAATTCCGCAGATAGTCGTTTATGAGTTCTTATGGGTTTTAGCTAAACTTACATCAGATGTTT
CCTTAATAAAAACTAAGATTGAAGAGTTAAGAGAGTTTGAAATTATATGCGAAAACCCGGAAACCATACTCAATGGTGTA
AAAATGTTAAAGGAAGACGGTAAACCTTTAAAGATGTTAAATGATTACGTTATTCTAGCCTTAGCTAAGGAATTAAAAGG
AGATTTAGCAACCTATGATGAAAAATTAAGGAAAATTGCTGAAAAACATGGTGTAAGAATAATTCCTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8668.92 Da
• Isoelectric Point: 5.1486

>AT23751 WP_012710994.1 NC_012588:c895082-894855 [Sulfolobus islandicus M.14.25]
MKVKVTRNFQVTIPSEIREKLGIKEGDYVEVTLDESNGTMIIKPYIRKWTTIRLNKKVDQEDIDKAVEEALNDSS

Nucleotide

• Length: 228 bp

>AT23751 NC_012588:c895082-894855 [Sulfolobus islandicus M.14.25]
ATGAAAGTAAAGGTTACTAGAAACTTTCAAGTTACTATACCCTCAGAGATAAGAGAAAAATTAGGTATAAAGGAAGGAGA
TTATGTCGAAGTAACTTTGGATGAAAGTAATGGAACTATGATAATTAAGCCGTATATAAGAAAGTGGACTACAATAAGGT
TAAATAAAAAAGTAGACCAAGAGGATATAGATAAGGCAGTTGAGGAGGCACTAAATGATAGTAGTTGA

Structures

Toxin

• AlphaFold DB: C4KG62

Antitoxin

• AlphaFold DB: M9UC56