Detailed information of TA system TA237507

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2465542..2466343
• Replicon: chromosome
• Accession: NZ_CP093011
• Organism: Escherichia coli strain 131

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D8EBK0
• Locus tag: LJF86_RS12140
• Protein ID: WP_001094436.1
• Coordinates: 2465966..2466343 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MN76
• Locus tag: LJF86_RS12135
• Protein ID: WP_015953067.1
• Coordinates: 2465542..2465919 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF86_RS12100 (2461454)	2461454..2462134	+	681	WP_001282927.1	WYL domain-containing protein	-
LJF86_RS12105 (2462282)	2462282..2462959	+	678	WP_001097312.1	hypothetical protein	-
LJF86_RS12110 (2462965)	2462965..2463198	+	234	WP_001278287.1	DUF905 family protein	-
LJF86_RS12115 (2463288)	2463288..2464106	+	819	WP_001175142.1	DUF932 domain-containing protein	-
LJF86_RS12120 (2464197)	2464197..2464682	+	486	WP_000860054.1	antirestriction protein	-
LJF86_RS12125 (2464697)	2464697..2465173	+	477	WP_001186756.1	RadC family protein	-
LJF86_RS12130 (2465242)	2465242..2465463	+	222	WP_000692312.1	DUF987 domain-containing protein	-
LJF86_RS12135 (2465542)	2465542..2465919	+	378	WP_015953067.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LJF86_RS12140 (2465966)	2465966..2466343	+	378	WP_001094436.1	TA system toxin CbtA family protein	Toxin
LJF86_RS12145 (2466340)	2466340..2466828	+	489	WP_000761714.1	DUF5983 family protein	-
LJF86_RS12150 (2466840)	2466840..2467037	+	198	WP_000839254.1	DUF957 domain-containing protein	-
LJF86_RS12155 (2467122)	2467122..2467967	+	846	WP_001280513.1	DUF4942 domain-containing protein	-
LJF86_RS12160 (2468038)	2468038..2469573	+	1536	WP_000492897.1	EAL domain-containing protein	-
LJF86_RS12165 (2469957)	2469957..2470116	+	160	Protein_2387	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13958.84 Da
• Isoelectric Point: 6.8603

>T237507 WP_001094436.1 NZ_CP093011:2465966-2466343 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T237507 NZ_LR882496:1970683-1970931 [Mycobacterium tuberculosis variant microti]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.60 Da
• Isoelectric Point: 6.2021

>AT237507 WP_015953067.1 NZ_CP093011:2465542-2465919 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT237507 NZ_LR882496:1970471-1970683 [Mycobacterium tuberculosis variant microti]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0E2L1N0

Antitoxin

• AlphaFold DB: A0A5Z3CIS0