Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2352946..2353522 Replicon chromosome
Accession NZ_CP093011
Organism Escherichia coli strain 131

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LJF86_RS11600 Protein ID WP_001297643.1
Coordinates 2353235..2353522 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LJF86_RS11595 Protein ID WP_000063156.1
Coordinates 2352946..2353248 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF86_RS11580 (2349542) 2349542..2351692 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LJF86_RS11585 (2351742) 2351742..2351945 + 204 WP_000467859.1 YbdD/YjiX family protein -
LJF86_RS11590 (2351956) 2351956..2352912 + 957 WP_001297640.1 GTPase -
LJF86_RS11595 (2352946) 2352946..2353248 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LJF86_RS11600 (2353235) 2353235..2353522 - 288 WP_001297643.1 BrnT family toxin Toxin
LJF86_RS11605 (2353721) 2353721..2354418 - 698 Protein_2274 IS1 family transposase -
LJF86_RS11610 (2354475) 2354475..2354570 + 96 Protein_2275 dienelactone hydrolase family protein -
LJF86_RS11615 (2354716) 2354716..2355948 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
LJF86_RS11620 (2355989) 2355989..2357269 + 1281 Protein_2277 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2353721..2353930 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T237506 WP_001297643.1 NZ_CP093011:c2353522-2353235 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T237506 NZ_LR882496:c1950128-1949739 [Mycobacterium tuberculosis variant microti]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTATCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT237506 WP_000063156.1 NZ_CP093011:c2353248-2352946 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT237506 NZ_LR882496:c1950352-1950125 [Mycobacterium tuberculosis variant microti]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References