Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2243235..2243785 Replicon chromosome
Accession NZ_CP093011
Organism Escherichia coli strain 131

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag LJF86_RS11055 Protein ID WP_001160966.1
Coordinates 2243235..2243549 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag LJF86_RS11060 Protein ID WP_000125566.1
Coordinates 2243552..2243785 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF86_RS11030 (2238910) 2238910..2240196 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
LJF86_RS11035 (2240196) 2240196..2241185 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
LJF86_RS11040 (2241182) 2241182..2242003 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
LJF86_RS11045 (2242006) 2242006..2242383 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
LJF86_RS11050 (2242390) 2242390..2243238 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
LJF86_RS11055 (2243235) 2243235..2243549 - 315 WP_001160966.1 CcdB family protein Toxin
LJF86_RS11060 (2243552) 2243552..2243785 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LJF86_RS11065 (2243871) 2243871..2244350 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
LJF86_RS11070 (2244542) 2244542..2246404 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
LJF86_RS11075 (2246397) 2246397..2246927 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
LJF86_RS11080 (2247035) 2247035..2248366 - 1332 WP_001183207.1 MFS transporter -
LJF86_RS11085 (2248425) 2248425..2248712 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T237505 WP_001160966.1 NZ_CP093011:c2243549-2243235 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T237505 NZ_LR882496:1776783-1777187 [Mycobacterium tuberculosis variant microti]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT237505 WP_000125566.1 NZ_CP093011:c2243785-2243552 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT237505 NZ_LR882496:1776574-1776777 [Mycobacterium tuberculosis variant microti]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References