Detailed information of TA system TA023750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 809445..810051
• Replicon: chromosome
• Accession: NC_012588
• Organism: Sulfolobus islandicus M.14.25

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C3MWJ7
• Locus tag: M1425_RS04230
• Protein ID: WP_012710924.1
• Coordinates: 809659..810051 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C4KFX1
• Locus tag: M1425_RS04225
• Protein ID: WP_012710923.1
• Coordinates: 809445..809669 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1425_RS04215 (M1425_0852)	806078..806842	+	765	WP_012710921.1	enoyl-CoA hydratase/isomerase family protein	-
M1425_RS04220 (M1425_0853)	806845..809112	+	2268	WP_012710922.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
M1425_RS04225 (M1425_0854)	809445..809669	+	225	WP_012710923.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
M1425_RS04230 (M1425_0855)	809659..810051	+	393	WP_012710924.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1425_RS04235 (M1425_0856)	810330..811430	+	1101	WP_012710760.1	ISNCY-like element ISSis4 family transposase	-
M1425_RS04240 (M1425_0857)	811903..812391	+	489	WP_012710925.1	hypothetical protein	-
M1425_RS04245 (M1425_0858)	812393..813328	+	936	WP_012710926.1	type I-A CRISPR-associated protein Cas7/Csa2	-
M1425_RS04250 (M1425_0859)	813329..814120	+	792	WP_012710927.1	type I-A CRISPR-associated protein Cas5a	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	810330..811430	1100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (23-118)

Antitoxin

• Post-segregation antitoxin CcdA (5-54)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15005.69 Da
• Isoelectric Point: 8.3342

>T23750 WP_012710924.1 NC_012588:809659-810051 [Sulfolobus islandicus M.14.25]
MKDKEFLFDASALYSLLDYVDKVDVKKIHILTLTFYEVGNVIWKEHYIHKKVKDPITLSILFHKLMRKLNVVEDPPLDGV
MRIAVERGLTYYDASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM

Nucleotide

• Length: 393 bp

>T23750 NC_012588:809659-810051 [Sulfolobus islandicus M.14.25]
ATGAAAGATAAGGAATTCCTATTCGATGCCTCAGCTTTATATTCGCTTTTAGATTACGTGGATAAGGTAGATGTTAAGAA
AATTCATATACTTACCCTAACTTTTTATGAGGTAGGTAACGTCATATGGAAGGAGCATTATATACACAAAAAGGTTAAAG
ATCCTATAACTCTTTCGATACTTTTCCATAAATTAATGCGAAAACTTAACGTAGTAGAGGATCCGCCTCTTGATGGGGTA
ATGAGAATTGCCGTAGAAAGGGGTTTAACTTATTATGACGCATCTTATGCGTATGTTGCTGAGTCTTTAGGGCTTATCCT
AGTGTCTAATGATAAAGAGCTGATAAGAAAAGCTAATGCCATTTCGTTAAAAGATTTGATAAAAAGTATGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8796.18 Da
• Isoelectric Point: 5.3410

>AT23750 WP_012710923.1 NC_012588:809445-809669 [Sulfolobus islandicus M.14.25]
MSDVISVRVKKELKKKAEELGINIREVVEKALEEAIKEKEKEELKDIAMRIKELMRDVSENDWVRAVRESRDER

Nucleotide

• Length: 225 bp

>AT23750 NC_012588:809445-809669 [Sulfolobus islandicus M.14.25]
ATGTCTGACGTAATAAGCGTAAGAGTGAAGAAAGAGTTGAAGAAGAAGGCAGAGGAGTTGGGAATTAACATTAGGGAAGT
TGTAGAAAAAGCTTTAGAGGAAGCCATAAAGGAGAAGGAGAAGGAAGAGCTTAAGGATATAGCTATGAGAATTAAGGAAC
TAATGAGGGATGTAAGTGAAAATGATTGGGTAAGGGCTGTTAGGGAGAGTAGAGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: C3MWJ7

Antitoxin

• AlphaFold DB: C4KFX1