Detailed information of TA system TA023749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 697191..698170
• Replicon: chromosome
• Accession: NC_012588
• Organism: Sulfolobus islandicus M.14.25

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C4KFP3
• Locus tag: M1425_RS03685
• Protein ID: WP_012710837.1
• Coordinates: 697191..697805 (-)
• Length: 205 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C3MWA9
• Locus tag: M1425_RS03690
• Protein ID: WP_012710838.1
• Coordinates: 697823..698170 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1425_RS03650 (M1425_0741)	692437..693627	-	1191	WP_012710830.1	amidohydrolase family protein	-
M1425_RS15420	693676..693875	-	200	Protein_753	hypothetical protein	-
M1425_RS03655 (M1425_0742)	693967..694566	-	600	WP_012710831.1	DUF2249 domain-containing protein	-
M1425_RS03660 (M1425_0743)	694815..695042	+	228	WP_012710832.1	hypothetical protein	-
M1425_RS03665 (M1425_0744)	695023..695346	-	324	WP_012710833.1	hypothetical protein	-
M1425_RS03670 (M1425_0745)	695350..696114	-	765	WP_012710834.1	plastocyanin/azurin family copper-binding protein	-
M1425_RS03675 (M1425_0746)	696219..696746	-	528	WP_012710835.1	hemerythrin domain-containing protein	-
M1425_RS03680 (M1425_0747)	696871..697146	+	276	WP_012710836.1	hypothetical protein	-
M1425_RS03685 (M1425_0748)	697191..697805	-	615	WP_012710837.1	helix-turn-helix domain-containing protein	Toxin
M1425_RS03690 (M1425_0749)	697823..698170	-	348	WP_012710838.1	DUF488 domain-containing protein	Antitoxin
M1425_RS03695	698239..698469	-	231	WP_012735723.1	C2H2-type zinc finger protein	-
M1425_RS14255	698539..698948	+	410	Protein_763	IS630 family transposase	-
M1425_RS03700 (M1425_0752)	699291..699779	+	489	WP_016730613.1	nucleotidyltransferase domain-containing protein	-
M1425_RS03705	699769..699993	+	225	WP_016730614.1	HEPN domain-containing protein	-
M1425_RS03710	699994..700206	+	213	WP_016730615.1	HEPN domain-containing protein	-
M1425_RS03715 (M1425_0754)	700703..701086	+	384	WP_016730616.1	metal-sulfur cluster assembly factor	-
M1425_RS03720 (M1425_0755)	701079..701510	-	432	WP_012710841.1	universal stress protein	-
M1425_RS03725 (M1425_0756)	701971..702909	-	939	WP_012710842.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (145-197)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 205 a.a.
• Molecular weight: 23169.24 Da
• Isoelectric Point: 9.5043

>T23749 WP_012710837.1 NC_012588:c697805-697191 [Sulfolobus islandicus M.14.25]
MLKSPFEVTILIEDHPCEVMRIISSTGLKATVGNVKLGDNTTDHIVLFEKEVQKDDLIKLKSHSVKVLKLNDSKVWVRTN
GCSVCKILYTSNVVIEKIKVIKERTLLYTLLVPNTIAFKEFLANLTSQGIKFTVLSTSEISSNDLTDRQMEILKLAYKMG
YFDDDRRVTLTELAKQLGISAPTLEEIMRRALRKVVKYYLDKVG

Nucleotide

• Length: 615 bp

>T23749 NC_012588:c697805-697191 [Sulfolobus islandicus M.14.25]
ATGTTAAAATCACCATTCGAAGTAACAATCTTGATTGAAGATCATCCTTGTGAGGTTATGAGGATAATATCTAGTACTGG
GCTTAAAGCAACCGTTGGCAACGTTAAACTCGGCGATAATACAACTGATCACATAGTCCTTTTCGAAAAAGAGGTTCAAA
AAGATGATCTGATTAAACTTAAGTCCCATAGTGTCAAAGTCTTAAAGCTTAATGATAGCAAAGTATGGGTTAGAACTAAT
GGATGCTCAGTATGTAAGATTCTCTATACTTCAAATGTTGTCATAGAGAAAATAAAAGTAATAAAAGAGAGAACATTACT
CTATACGCTTTTGGTCCCTAATACGATAGCTTTTAAGGAGTTTCTAGCTAATCTTACTAGTCAAGGGATAAAGTTTACAG
TCCTAAGCACCTCTGAAATAAGCAGTAATGACTTAACTGATAGACAAATGGAAATATTGAAACTTGCATACAAGATGGGT
TATTTTGATGATGATAGGAGGGTAACTTTAACTGAATTAGCAAAGCAATTGGGAATATCAGCTCCGACTTTGGAGGAAAT
TATGAGGAGAGCGTTAAGGAAAGTCGTCAAGTATTATTTAGACAAAGTAGGGTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13731.06 Da
• Isoelectric Point: 9.9999

>AT23749 WP_012710838.1 NC_012588:c698170-697823 [Sulfolobus islandicus M.14.25]
MIKIKRVYDPIEKDDGIRILVDRLWPRGIGKNKVDLWLKDIAPSDELRKWYGHDPNKWDEFKRKYFEELDANPKMSILLQ
LIRKGENITLLYASKSPYNNAVALKEYLEKKILKQ

Nucleotide

• Length: 348 bp

>AT23749 NC_012588:c698170-697823 [Sulfolobus islandicus M.14.25]
ATGATTAAGATAAAGAGGGTTTACGATCCAATAGAGAAAGATGACGGAATAAGAATCTTAGTGGATAGGTTATGGCCAAG
AGGAATTGGTAAAAATAAGGTAGATTTATGGCTTAAAGATATAGCACCTAGTGATGAGTTACGAAAATGGTACGGTCATG
ATCCTAATAAATGGGATGAGTTCAAAAGGAAATATTTTGAGGAACTAGATGCTAATCCAAAGATGAGCATTTTATTACAA
TTAATTAGAAAGGGCGAAAATATTACCTTACTTTACGCCTCCAAATCACCGTATAATAATGCGGTTGCGTTAAAGGAGTA
TCTAGAAAAAAAGATATTAAAACAATAG

Structures

Toxin

• AlphaFold DB: C4KFP3

Antitoxin

• AlphaFold DB: C3MWA9