Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5294089..5294665 Replicon chromosome
Accession NZ_CP093010
Organism Klebsiella michiganensis strain K342

Toxin (Protein)


Gene name shpA Uniprot ID A0A1D8JNH2
Locus tag MMY94_RS25255 Protein ID WP_042933975.1
Coordinates 5294378..5294665 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag MMY94_RS25250 Protein ID WP_025108476.1
Coordinates 5294089..5294391 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMY94_RS25235 (MMY94_25235) 5290930..5291265 + 336 WP_014227760.1 endoribonuclease SymE -
MMY94_RS25240 (MMY94_25240) 5291717..5292628 + 912 WP_032693024.1 acetamidase/formamidase family protein -
MMY94_RS25245 (MMY94_25245) 5292625..5293968 + 1344 WP_032693026.1 APC family permease -
MMY94_RS25250 (MMY94_25250) 5294089..5294391 - 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
MMY94_RS25255 (MMY94_25255) 5294378..5294665 - 288 WP_042933975.1 BrnT family toxin Toxin
MMY94_RS25260 (MMY94_25260) 5294916..5295359 - 444 WP_032693030.1 FosA family fosfomycin resistance glutathione transferase -
MMY94_RS25265 (MMY94_25265) 5295353..5296261 - 909 WP_032693031.1 LysR family transcriptional regulator -
MMY94_RS25270 (MMY94_25270) 5296349..5297131 + 783 WP_032693033.1 NAD(P)H-dependent oxidoreductase -
MMY94_RS25275 (MMY94_25275) 5297279..5297863 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
MMY94_RS25280 (MMY94_25280) 5298009..5298809 + 801 WP_032693034.1 winged helix-turn-helix domain-containing protein -
MMY94_RS25285 (MMY94_25285) 5298806..5299324 + 519 WP_004098240.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11239.69 Da        Isoelectric Point: 8.5899

>T237488 WP_042933975.1 NZ_CP093010:c5294665-5294378 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T237488 NZ_LR882496:712681-713082 [Mycobacterium tuberculosis variant microti]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT237488 WP_025108476.1 NZ_CP093010:c5294391-5294089 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT237488 NZ_LR882496:712457-712684 [Mycobacterium tuberculosis variant microti]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGTCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JNH2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References