Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4602748..4603367 Replicon chromosome
Accession NZ_CP093010
Organism Klebsiella michiganensis strain K342

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag MMY94_RS22060 Protein ID WP_004099646.1
Coordinates 4603149..4603367 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag MMY94_RS22055 Protein ID WP_004129911.1
Coordinates 4602748..4603122 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMY94_RS22045 (MMY94_22045) 4597905..4599098 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MMY94_RS22050 (MMY94_22050) 4599121..4602267 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
MMY94_RS22055 (MMY94_22055) 4602748..4603122 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
MMY94_RS22060 (MMY94_22060) 4603149..4603367 + 219 WP_004099646.1 HHA domain-containing protein Toxin
MMY94_RS22065 (MMY94_22065) 4603530..4604096 + 567 WP_032694159.1 maltose O-acetyltransferase -
MMY94_RS22070 (MMY94_22070) 4604068..4604202 - 135 WP_223226764.1 hypothetical protein -
MMY94_RS22075 (MMY94_22075) 4604223..4604693 + 471 WP_014228205.1 YlaC family protein -
MMY94_RS22080 (MMY94_22080) 4604668..4606122 - 1455 WP_032694158.1 PLP-dependent aminotransferase family protein -
MMY94_RS22085 (MMY94_22085) 4606224..4606922 + 699 WP_014228203.1 GNAT family protein -
MMY94_RS22090 (MMY94_22090) 4606919..4607059 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MMY94_RS22095 (MMY94_22095) 4607059..4607322 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T237486 WP_004099646.1 NZ_CP093010:4603149-4603367 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T237486 NZ_LR882496:c699242-698829 [Mycobacterium tuberculosis variant microti]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT237486 WP_004129911.1 NZ_CP093010:4602748-4603122 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT237486 NZ_LR882496:c699475-699239 [Mycobacterium tuberculosis variant microti]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References