Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2056503..2057093 Replicon chromosome
Accession NZ_CP093010
Organism Klebsiella michiganensis strain K342

Toxin (Protein)


Gene name pemK Uniprot ID A0A1D8JW90
Locus tag MMY94_RS09790 Protein ID WP_032693748.1
Coordinates 2056761..2057093 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag MMY94_RS09785 Protein ID WP_004852307.1
Coordinates 2056503..2056760 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMY94_RS09775 (MMY94_09775) 2055579..2056052 + 474 WP_050595132.1 hypothetical protein -
MMY94_RS09780 (MMY94_09780) 2056049..2056242 + 194 Protein_1917 helix-turn-helix domain-containing protein -
MMY94_RS09785 (MMY94_09785) 2056503..2056760 + 258 WP_004852307.1 antitoxin Antitoxin
MMY94_RS09790 (MMY94_09790) 2056761..2057093 + 333 WP_032693748.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MMY94_RS09800 (MMY94_09800) 2057416..2058873 + 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
MMY94_RS09810 (MMY94_09810) 2059837..2061291 - 1455 WP_014229909.1 AMP nucleosidase -
MMY94_RS09815 (MMY94_09815) 2061424..2061681 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11752.67 Da        Isoelectric Point: 10.4751

>T237477 WP_032693748.1 NZ_CP093010:2056761-2057093 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLARLPVVVPITSGGKFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMVARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T237477 NZ_LR882494:c71810-71652 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT237477 WP_004852307.1 NZ_CP093010:2056503-2056760 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT237477 NZ_LR882494:c72078-71854 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JW90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References