Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 898316..898973 Replicon chromosome
Accession NZ_CP093010
Organism Klebsiella michiganensis strain K342

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag MMY94_RS04400 Protein ID WP_004854060.1
Coordinates 898563..898973 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag MMY94_RS04395 Protein ID WP_004124953.1
Coordinates 898316..898582 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMY94_RS04380 (MMY94_04380) 893550..893975 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
MMY94_RS04385 (MMY94_04385) 894096..896894 - 2799 WP_014226794.1 transcriptional regulator DagR -
MMY94_RS04390 (MMY94_04390) 897088..898071 - 984 WP_014226793.1 tRNA-modifying protein YgfZ -
MMY94_RS04395 (MMY94_04395) 898316..898582 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
MMY94_RS04400 (MMY94_04400) 898563..898973 + 411 WP_004854060.1 protein YgfX Toxin
MMY94_RS04405 (MMY94_04405) 898982..899503 - 522 WP_014226792.1 flavodoxin FldB -
MMY94_RS04410 (MMY94_04410) 899625..900521 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
MMY94_RS04415 (MMY94_04415) 900544..901257 + 714 WP_032693807.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MMY94_RS04420 (MMY94_04420) 901263..902996 + 1734 WP_032693806.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T237476 WP_004854060.1 NZ_CP093010:898563-898973 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T237476 NZ_LR882494:c53857-53576 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT237476 WP_004124953.1 NZ_CP093010:898316-898582 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT237476 NZ_LR882494:c54123-53854 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References