Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56987..57534 Replicon chromosome
Accession NZ_CP093010
Organism Klebsiella michiganensis strain K342

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag MMY94_RS00285 Protein ID WP_014227299.1
Coordinates 56987..57295 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag MMY94_RS00290 Protein ID WP_014227298.1
Coordinates 57298..57534 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMY94_RS00260 (MMY94_00260) 52622..52933 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
MMY94_RS00265 (MMY94_00265) 53228..54169 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
MMY94_RS00270 (MMY94_00270) 54193..54486 - 294 WP_014227302.1 YicS family protein -
MMY94_RS00275 (MMY94_00275) 54696..55673 + 978 WP_032695133.1 Rpn family recombination-promoting nuclease/putative transposase -
MMY94_RS28540 55805..55930 + 126 WP_256557856.1 hypothetical protein -
MMY94_RS00280 (MMY94_00280) 55934..56836 - 903 WP_014227300.1 EamA family transporter -
MMY94_RS00285 (MMY94_00285) 56987..57295 - 309 WP_014227299.1 CcdB family protein Toxin
MMY94_RS00290 (MMY94_00290) 57298..57534 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MMY94_RS00295 (MMY94_00295) 57640..59073 - 1434 WP_014227297.1 PTS N-acetylmuramic acid transporter subunit IIBC -
MMY94_RS00300 (MMY94_00300) 59098..60000 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
MMY94_RS00305 (MMY94_00305) 60161..61327 - 1167 WP_014227296.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T237474 WP_014227299.1 NZ_CP093010:c57295-56987 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T237474 NZ_LR882493:c4351200-4351027 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT237474 WP_014227298.1 NZ_CP093010:c57534-57298 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT237474 NZ_LR882493:c4351524-4351228 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References