Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 42819..43384 Replicon plasmid pLiSL46
Accession NZ_CP092988
Organism Leptospira interrogans strain FMAS_KG2

Toxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag FH588_RS22710 Protein ID WP_000237066.1
Coordinates 42819..43142 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag FH588_RS22715 Protein ID WP_000648189.1
Coordinates 43136..43384 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH588_RS22680 (FH588_22675) 38151..39461 + 1311 WP_000801548.1 IS5 family transposase -
FH588_RS22685 (FH588_22680) 39859..40737 + 879 Protein_29 kelch repeat-containing protein -
FH588_RS22690 (FH588_22685) 41122..41313 - 192 Protein_30 AraC family transcriptional regulator -
FH588_RS22695 (FH588_22690) 41606..42367 - 762 Protein_31 hypothetical protein -
FH588_RS22700 (FH588_22695) 42283..42462 + 180 WP_000536132.1 hypothetical protein -
FH588_RS22705 (FH588_22700) 42526..42711 - 186 Protein_33 toxin-antitoxin system, toxin component -
FH588_RS22710 (FH588_22705) 42819..43142 - 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH588_RS22715 (FH588_22710) 43136..43384 - 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH588_RS22720 (FH588_22715) 44564..45862 + 1299 WP_061234536.1 group II intron reverse transcriptase/maturase -
FH588_RS22725 (FH588_22720) 46597..46977 + 381 WP_241468674.1 hypothetical protein -
FH588_RS22730 (FH588_22725) 47249..48223 + 975 WP_241468675.1 Rpn family recombination-promoting nuclease/putative transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..76132 76132


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-105)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>T237471 WP_000237066.1 NZ_CP092988:c43142-42819 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>T237471 NZ_LR882493:3552127-3552345 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>AT237471 WP_000648189.1 NZ_CP092988:c43384-43136 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>AT237471 NZ_LR882493:3551727-3552101 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

References