Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 34678..35249 Replicon plasmid pLiSL44
Accession NZ_CP092986
Organism Leptospira interrogans strain FMAS_KG2

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag FH588_RS22280 Protein ID WP_000240418.1
Coordinates 34678..35019 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag FH588_RS22285 Protein ID WP_000443825.1
Coordinates 35013..35249 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH588_RS22245 (FH588_22240) 29995..30456 - 462 WP_241468668.1 hypothetical protein -
FH588_RS22250 (FH588_22245) 30808..31955 + 1148 WP_241468669.1 IS3-like element IS1500B family transposase -
FH588_RS22255 (FH588_22250) 32207..32407 - 201 WP_001206464.1 hypothetical protein -
FH588_RS22260 (FH588_22255) 32831..33556 + 726 WP_239033801.1 hypothetical protein -
FH588_RS22265 (FH588_22260) 33559..33900 + 342 WP_000727287.1 hypothetical protein -
FH588_RS22270 (FH588_22265) 33961..34227 + 267 WP_000844450.1 hypothetical protein -
FH588_RS22275 (FH588_22270) 34227..34610 + 384 WP_000617796.1 hypothetical protein -
FH588_RS22280 (FH588_22275) 34678..35019 - 342 WP_000240418.1 endoribonuclease MazF Toxin
FH588_RS22285 (FH588_22280) 35013..35249 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH588_RS22290 (FH588_22285) 35598..36389 - 792 WP_061243661.1 hypothetical protein -
FH588_RS22295 (FH588_22290) 37212..38510 + 1299 WP_061234536.1 group II intron reverse transcriptase/maturase -
FH588_RS22300 (FH588_22295) 39239..39688 + 450 WP_240739037.1 leucine-rich repeat domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..70292 70292


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T237470 WP_000240418.1 NZ_CP092986:c35019-34678 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T237470 NZ_LR882493:c2747102-2746851 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT237470 WP_000443825.1 NZ_CP092986:c35249-35013 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT237470 NZ_LR882493:c2746854-2746600 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References