Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1859562..1860138 Replicon chromosome
Accession NZ_CP092985
Organism Leptospira interrogans strain FMAS_KG2

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FH588_RS09950 Protein ID WP_000617906.1
Coordinates 1859797..1860138 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH588_RS09945 Protein ID WP_000844758.1
Coordinates 1859562..1859810 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH588_RS09930 (FH588_09930) 1855222..1857204 + 1983 WP_000802798.1 GAF domain-containing protein -
FH588_RS09935 (FH588_09935) 1857838..1858296 - 459 WP_000453264.1 hypothetical protein -
FH588_RS09940 (FH588_09940) 1858450..1858746 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH588_RS09945 (FH588_09945) 1859562..1859810 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH588_RS09950 (FH588_09950) 1859797..1860138 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH588_RS09955 (FH588_09955) 1861163..1862155 - 993 WP_000041092.1 aldolase/citrate lyase family protein -
FH588_RS09960 (FH588_09960) 1862295..1863230 - 936 WP_000004530.1 ornithine carbamoyltransferase -
FH588_RS09965 (FH588_09965) 1864034..1864501 + 468 WP_000060052.1 hypothetical protein -
FH588_RS09970 (FH588_09970) 1864498..1864902 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T237469 WP_000617906.1 NZ_CP092985:1859797-1860138 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T237469 NZ_LR882493:c2746854-2746600 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT237469 WP_000844758.1 NZ_CP092985:1859562-1859810 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT237469 NZ_LR882493:c2747102-2746851 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References