Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 1433554..1434182 Replicon chromosome
Accession NZ_CP092985
Organism Leptospira interrogans strain FMAS_KG2

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH588_RS08335 Protein ID WP_000281042.1
Coordinates 1433554..1433952 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag FH588_RS08340 Protein ID WP_001192482.1
Coordinates 1433952..1434182 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH588_RS08300 (FH588_08300) 1429070..1429912 + 843 WP_137170910.1 hypothetical protein -
FH588_RS08305 (FH588_08305) 1430168..1431245 - 1078 Protein_1178 hypothetical protein -
FH588_RS08310 (FH588_08310) 1431356..1431616 - 261 WP_137108817.1 hypothetical protein -
FH588_RS08315 (FH588_08315) 1431638..1431937 - 300 Protein_1180 hypothetical protein -
FH588_RS08320 (FH588_08320) 1432036..1432308 - 273 WP_240738869.1 BrnA antitoxin family protein -
FH588_RS08325 (FH588_08325) 1432295..1432588 - 294 WP_061243121.1 BrnT family toxin -
FH588_RS08330 (FH588_08330) 1432768..1433298 - 531 WP_061261813.1 hypothetical protein -
FH588_RS08335 (FH588_08335) 1433554..1433952 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH588_RS08340 (FH588_08340) 1433952..1434182 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH588_RS08345 (FH588_08345) 1434550..1434894 - 345 WP_000392070.1 hypothetical protein -
FH588_RS08350 (FH588_08350) 1435117..1435794 - 678 WP_000071998.1 hypothetical protein -
FH588_RS08355 (FH588_08355) 1436026..1436715 - 690 WP_000590462.1 SH3 domain-containing protein -
FH588_RS08360 (FH588_08360) 1436941..1437864 - 924 WP_000680711.1 M28 family peptidase -
FH588_RS08365 (FH588_08365) 1438071..1438700 - 630 WP_001081629.1 S24 family peptidase -
FH588_RS08370 (FH588_08370) 1438832..1439041 + 210 WP_000041838.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T237468 WP_000281042.1 NZ_CP092985:c1433952-1433554 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T237468 NZ_LR882493:c2578354-2578251 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT237468 WP_001192482.1 NZ_CP092985:c1434182-1433952 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT237468 NZ_LR882493:2578213-2578484 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References