Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 44093..44694 Replicon plasmid pTEM135_005215
Accession NZ_CP092976
Organism Escherichia coli strain WCHEC005215

Toxin (Protein)


Gene name doc Uniprot ID A0A5U0JPI4
Locus tag MM176_RS24365 Protein ID WP_024166788.1
Coordinates 44093..44473 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A5T4YUW9
Locus tag MM176_RS24370 Protein ID WP_033545779.1
Coordinates 44473..44694 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM176_RS24350 (MM176_24380) 40768..41710 + 943 Protein_21 IS3 family transposase -
MM176_RS24355 (MM176_24385) 41737..42433 - 697 Protein_22 IS1 family transposase -
MM176_RS24360 (MM176_24390) 42827..43855 + 1029 WP_072851570.1 tyrosine-type recombinase/integrase -
MM176_RS24365 (MM176_24395) 44093..44473 - 381 WP_024166788.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MM176_RS24370 (MM176_24400) 44473..44694 - 222 WP_033545779.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
MM176_RS24375 (MM176_24405) 44767..45159 - 393 WP_000506718.1 S24 family peptidase -
MM176_RS24380 (MM176_24410) 45271..45519 - 249 WP_001286530.1 DNA polymerase III subunit theta -
MM176_RS24385 (MM176_24415) 45522..45788 - 267 WP_000946114.1 hypothetical protein -
MM176_RS24390 (MM176_24420) 45858..46148 - 291 WP_048659782.1 hypothetical protein -
MM176_RS24395 (MM176_24430) 46276..46980 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
MM176_RS24400 (MM176_24435) 47033..49246 + 2214 Protein_31 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / blaTEM-1B / dfrA14 / qnrS1 / floR - 1..122037 122037
- inside IScluster/Tn - - 40768..49246 8478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13663.58 Da        Isoelectric Point: 5.6406

>T237462 WP_024166788.1 NZ_CP092976:c44473-44093 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T237462 NZ_LR882493:997456-997791 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT237462 WP_033545779.1 NZ_CP092976:c44694-44473 [Escherichia coli]
MQSINFRTARGNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT237462 NZ_LR882493:997208-997456 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5U0JPI4

Antitoxin

Source ID Structure
AlphaFold DB A0A5T4YUW9

References