Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 45330..45855 Replicon plasmid pCTXM14_005215
Accession NZ_CP092974
Organism Escherichia coli strain WCHEC005215

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MM176_RS23550 Protein ID WP_001159871.1
Coordinates 45550..45855 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MM176_RS23545 Protein ID WP_000813634.1
Coordinates 45330..45548 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM176_RS23520 (MM176_23545) 40661..40951 + 291 WP_000648897.1 hypothetical protein -
MM176_RS23525 (MM176_23550) 41524..42525 + 1002 WP_000785695.1 DUF4238 domain-containing protein -
MM176_RS23530 (MM176_23555) 42699..43043 - 345 WP_000792769.1 hypothetical protein -
MM176_RS23535 (MM176_23560) 43541..43795 - 255 WP_000678528.1 hypothetical protein -
MM176_RS23540 (MM176_23565) 43845..44771 - 927 WP_001290414.1 hypothetical protein -
MM176_RS23545 (MM176_23570) 45330..45548 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MM176_RS23550 (MM176_23575) 45550..45855 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MM176_RS23555 (MM176_23580) 45856..46665 + 810 WP_000016963.1 site-specific integrase -
MM176_RS23560 (MM176_23585) 46837..47031 + 195 Protein_58 colicin M immunity protein -
MM176_RS23565 (MM176_23590) 47087..47784 + 698 WP_223200906.1 IS1-like element IS1A family transposase -
MM176_RS23570 (MM176_23595) 47800..48390 - 591 Protein_60 colicin-like bacteriocin tRNase domain-containing protein -
MM176_RS23575 (MM176_23600) 48570..48996 + 427 Protein_61 tyrosine-type recombinase/integrase -
MM176_RS23580 (MM176_23605) 49188..49463 - 276 WP_000239529.1 hypothetical protein -
MM176_RS23585 (MM176_23610) 49457..50101 - 645 WP_000633913.1 division plane positioning ATPase MipZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / cmlA1 / qacE / blaCTX-M-14 - 1..104665 104665


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T237461 WP_001159871.1 NZ_CP092974:45550-45855 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T237461 NZ_LR882493:855857-856264 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT237461 WP_000813634.1 NZ_CP092974:45330-45548 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT237461 NZ_LR882493:855610-855876 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References