Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 25567..26126 | Replicon | plasmid pCTXM14_005215 |
| Accession | NZ_CP092974 | ||
| Organism | Escherichia coli strain WCHEC005215 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | S1PMX5 |
| Locus tag | MM176_RS23435 | Protein ID | WP_000421257.1 |
| Coordinates | 25851..26126 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | MM176_RS23430 | Protein ID | WP_001178089.1 |
| Coordinates | 25567..25851 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MM176_RS23405 (MM176_23430) | 21242..21814 | + | 573 | Protein_27 | IS1380-like element ISEcp1 family transposase | - |
| MM176_RS23410 (MM176_23435) | 21871..22839 | - | 969 | WP_013362812.1 | IS5-like element IS903B family transposase | - |
| MM176_RS23415 (MM176_23440) | 22874..23749 | - | 876 | WP_001617865.1 | extended-spectrum class A beta-lactamase CTX-M-14 | - |
| MM176_RS23420 (MM176_23445) | 23999..24286 | - | 288 | Protein_30 | transposase | - |
| MM176_RS23425 (MM176_23450) | 24352..25056 | + | 705 | WP_001067845.1 | IS6-like element IS26 family transposase | - |
| MM176_RS23430 (MM176_23455) | 25567..25851 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| MM176_RS23435 (MM176_23460) | 25851..26126 | + | 276 | WP_000421257.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| MM176_RS23445 (MM176_23470) | 26938..27306 | + | 369 | Protein_35 | colicin-like pore-forming protein | - |
| MM176_RS23450 (MM176_23475) | 27328..27663 | - | 336 | WP_001080730.1 | colicin 1A immunity protein | - |
| MM176_RS23455 (MM176_23480) | 27792..28139 | + | 348 | WP_000142451.1 | DUF6404 family protein | - |
| MM176_RS23460 (MM176_23485) | 28226..28930 | - | 705 | WP_001067845.1 | IS6-like element IS26 family transposase | - |
| MM176_RS23465 (MM176_23490) | 28955..29479 | + | 525 | WP_050195267.1 | recombinase family protein | - |
| MM176_RS23470 (MM176_23495) | 29556..30947 | + | 1392 | WP_000085084.1 | SIR2 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | mph(A) / cmlA1 / qacE / blaCTX-M-14 | - | 1..104665 | 104665 | |
| - | inside | IScluster/Tn | mph(A) / cmlA1 / qacE / blaCTX-M-14 | - | 10243..36228 | 25985 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10749.45 Da Isoelectric Point: 9.9324
>T237460 WP_000421257.1 NZ_CP092974:25851-26126 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T237460 NZ_LR882493:718576-718872 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT237460 WP_001178089.1 NZ_CP092974:25567-25851 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT237460 NZ_LR882493:718874-719269 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1PMX5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |