Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4092657..4093233 Replicon chromosome
Accession NZ_CP092973
Organism Escherichia coli strain WCHEC005215

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag MM176_RS19690 Protein ID WP_001297643.1
Coordinates 4092946..4093233 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag MM176_RS19685 Protein ID WP_000063160.1
Coordinates 4092657..4092959 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM176_RS19670 (4089253) 4089253..4091403 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
MM176_RS19675 (4091453) 4091453..4091656 + 204 WP_000467859.1 YbdD/YjiX family protein -
MM176_RS19680 (4091667) 4091667..4092623 + 957 WP_001297640.1 GTPase -
MM176_RS19685 (4092657) 4092657..4092959 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
MM176_RS19690 (4092946) 4092946..4093233 - 288 WP_001297643.1 BrnT family toxin Toxin
MM176_RS25040 (4093432) 4093432..4094128 - 697 Protein_3876 IS1 family transposase -
MM176_RS19705 (4094443) 4094443..4095675 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
MM176_RS19710 (4095716) 4095716..4096996 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4093432..4093641 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T237457 WP_001297643.1 NZ_CP092973:c4093233-4092946 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T237457 NZ_LR882061:41685-41834 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT237457 WP_000063160.1 NZ_CP092973:c4092959-4092657 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT237457 NZ_LR882061:c41638-41580 [Escherichia coli]
AGATACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References