Detailed information of TA system TA237432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2695835..2697056
• Replicon: chromosome
• Accession: NZ_CP092972
• Organism: Pseudomonas aeruginosa strain PA01135

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MMG97_RS12750
• Protein ID: WP_003114949.1
• Coordinates: 2695835..2696413 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MMG97_RS12755
• Protein ID: WP_003088112.1
• Coordinates: 2696502..2697056 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMG97_RS12735 (MMG97_12725)	2691112..2692389	+	1278	WP_003102743.1	TolC family protein	-
MMG97_RS12740 (MMG97_12730)	2692379..2694550	+	2172	WP_003114950.1	type I secretion system permease/ATPase	-
MMG97_RS12745 (MMG97_12735)	2694540..2695727	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MMG97_RS12750 (MMG97_12740)	2695835..2696413	-	579	WP_003114949.1	HD domain-containing protein	Toxin
MMG97_RS12755 (MMG97_12745)	2696502..2697056	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
MMG97_RS12760 (MMG97_12750)	2697214..2699409	-	2196	WP_003114948.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
MMG97_RS12765 (MMG97_12755)	2699414..2699875	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
MMG97_RS12770 (MMG97_12760)	2700206..2700529	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
MMG97_RS12775 (MMG97_12765)	2700542..2700940	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
MMG97_RS12780 (MMG97_12770)	2700937..2701488	-	552	WP_003102727.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T237432 WP_003114949.1 NZ_CP092972:c2696413-2695835 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T237432 NZ_LR882057:291659-292186 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT237432 WP_003088112.1 NZ_CP092972:c2697056-2696502 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT237432 NZ_LR882057:291386-291652 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA