Detailed information of TA system TA023743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 958585..959120
• Replicon: plasmid pNGR234b
• Accession: NC_012586
• Organism: Sinorhizobium fredii NGR234

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: NGR_RS04645
• Protein ID: WP_015887076.1
• Coordinates: 958585..958860 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: NGR_RS04650
• Protein ID: WP_015887077.1
• Coordinates: 958857..959120 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGR_RS04610	953724..954221	+	498	WP_015887068.1	hypothetical protein	-
NGR_RS04615	954356..954649	+	294	WP_015887069.1	hypothetical protein	-
NGR_RS32590	955129..955602	+	474	WP_193377874.1	hypothetical protein	-
NGR_RS04630	956648..956809	-	162	WP_015887073.1	hypothetical protein	-
NGR_RS04635	956839..958009	-	1171	Protein_920	ATP-binding protein	-
NGR_RS04645	958585..958860	-	276	WP_015887076.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NGR_RS04650	958857..959120	-	264	WP_015887077.1	ribbon-helix-helix domain-containing protein	Antitoxin
NGR_RS04655	959572..959859	+	288	WP_164923877.1	transposase	-
NGR_RS04660	959856..960203	+	348	WP_015887079.1	IS66 family insertion sequence element accessory protein TnpB	-
NGR_RS04665	960282..961934	+	1653	WP_015887080.1	IS66 family transposase	-
NGR_RS04670	961937..962521	+	585	WP_015887081.1	plasmid pRiA4b ORF-3 family protein	-
NGR_RS04675	963007..963318	+	312	WP_015887083.1	hypothetical protein	-
NGR_RS04680	963428..963706	-	279	WP_015887084.1	BrnA antitoxin family protein	-
NGR_RS04685	963690..963992	-	303	WP_164923878.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / katA	1..2430033	2430033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10928.54 Da
• Isoelectric Point: 9.9707

>T23743 WP_015887076.1 NC_012586:c958860-958585 [Sinorhizobium fredii NGR234]
MKLEWTSKAVSDIGRLYDFLAPVNRQAAVRTVQSLTSAPTRLLEQPRIGERLDEFDPREVRRILIGHYEMRYEIRQSTIY
VLRLWHTREER

Nucleotide

• Length: 276 bp

>T23743 NC_012586:c958860-958585 [Sinorhizobium fredii NGR234]
ATGAAGCTTGAGTGGACGAGTAAAGCGGTGTCCGATATCGGGCGCCTCTACGATTTCCTGGCGCCAGTAAATCGGCAAGC
TGCTGTCCGAACCGTCCAATCATTGACAAGCGCTCCAACCCGCCTGCTTGAACAACCGCGGATCGGCGAGCGCCTGGACG
AGTTCGATCCCCGGGAGGTCCGCCGAATCCTCATTGGCCATTACGAAATGCGCTATGAGATCAGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAAGAGCGATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9816.14 Da
• Isoelectric Point: 5.6088

>AT23743 WP_015887077.1 NC_012586:c959120-958857 [Sinorhizobium fredii NGR234]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALAAWIDQEEERRRLTLEALADVDSGRVIHHQSVQAWADSLDSDN
PRPLPIS

Nucleotide

• Length: 264 bp

>AT23743 NC_012586:c959120-958857 [Sinorhizobium fredii NGR234]
ATGGAAACGAAGGTACTGACAGCGCACGTGCCGCTGCCGCTCGCCGAGAAAGTCGATCAAATCGCGGCTCGACTTGAGCG
TTCGCGCGGTTGGATCGTGAAGCAAGCGTTGGCGGCCTGGATCGACCAGGAAGAGGAACGCCGGCGTTTGACTCTGGAGG
CGCTCGCTGACGTGGACAGCGGCCGGGTAATCCACCACCAGTCCGTGCAGGCGTGGGCCGACAGTCTCGACAGCGATAAC
CCGCGACCGCTACCGATATCATGA