Detailed information of TA system TA237426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 189569..190203
• Replicon: plasmid pMpar-1
• Accession: NZ_CP092969
• Organism: Methylocystis parvus OBBP

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MMG94_RS20590
• Protein ID: WP_016919208.1
• Coordinates: 189569..189970 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B8M6T5
• Locus tag: MMG94_RS20595
• Protein ID: WP_026016140.1
• Coordinates: 189967..190203 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMG94_RS20565 (MMG94_20470)	185840..185929	+	90	Protein_172	flavin reductase family protein	-
MMG94_RS20570 (MMG94_20475)	186125..186619	-	495	WP_157212434.1	helix-turn-helix domain-containing protein	-
MMG94_RS20575 (MMG94_20480)	186950..187480	-	531	WP_154420419.1	hypothetical protein	-
MMG94_RS20580 (MMG94_20485)	188013..188357	+	345	WP_016919211.1	single-stranded DNA-binding protein	-
MMG94_RS20585 (MMG94_20490)	189085..189462	+	378	WP_016919209.1	single-stranded DNA-binding protein	-
MMG94_RS20590 (MMG94_20495)	189569..189970	-	402	WP_016919208.1	type II toxin-antitoxin system VapC family toxin	Toxin
MMG94_RS20595 (MMG94_20500)	189967..190203	-	237	WP_026016140.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MMG94_RS20600 (MMG94_20505)	190380..190763	-	384	WP_154420362.1	hypothetical protein	-
MMG94_RS20605 (MMG94_20510)	190795..191481	-	687	WP_016919205.1	P-loop NTPase	-
MMG94_RS20610 (MMG94_20515)	191563..191847	+	285	WP_154420364.1	hypothetical protein	-
MMG94_RS20615 (MMG94_20520)	192527..192931	+	405	WP_016919203.1	plasmid mobilization relaxosome protein MobC	-
MMG94_RS20620 (MMG94_20525)	193285..193611	-	327	WP_244415258.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..248224	248224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (10-54)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14467.87 Da
• Isoelectric Point: 7.8528

>T237426 WP_016919208.1 NZ_CP092969:c189970-189569 [Methylocystis parvus OBBP]
MTRYLLDTNIISDLIRNPKGKVAKHIARVGEKNVCTSIIVAAELRYGCAKSGSKRLLEAVELLLGELDVLSLEAPADAEY
GRIRAELERKGSPIGGNDLLIAAHALAIEATMVTANVDEFTRVKGLKVQNWLL

Nucleotide

• Length: 402 bp

>T237426 NZ_LR882053:c94044-93739 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9024.39 Da
• Isoelectric Point: 4.9062

>AT237426 WP_026016140.1 NZ_CP092969:c190203-189967 [Methylocystis parvus OBBP]
MGEARHVKLFRNGRNQAVRIPVEFELPGDEAIMRREGDRLVLEPTRKRGLLGLLETMEPLDETFPEIEDRPPAPEKIF

Nucleotide

• Length: 237 bp

>AT237426 NZ_LR882053:c94264-94046 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B8M6T5