Detailed information of TA system TA237424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2861646..2862316
• Replicon: chromosome
• Accession: NZ_CP092968
• Organism: Methylocystis parvus OBBP

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MMG94_RS13915
• Protein ID: WP_016918545.1
• Coordinates: 2861909..2862316 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B8M3R6
• Locus tag: MMG94_RS13910
• Protein ID: WP_016918546.1
• Coordinates: 2861646..2861900 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMG94_RS13890 (MMG94_13840)	2856860..2858554	+	1695	WP_040578912.1	lysine--tRNA ligase	-
MMG94_RS13895 (MMG94_13845)	2858554..2859102	+	549	WP_016918549.1	alpha/beta hydrolase	-
MMG94_RS13900 (MMG94_13850)	2859184..2859795	-	612	WP_016918548.1	L,D-transpeptidase	-
MMG94_RS13905 (MMG94_13855)	2859978..2861564	-	1587	WP_016918547.1	phosphoglycerate dehydrogenase	-
MMG94_RS13910 (MMG94_13860)	2861646..2861900	+	255	WP_016918546.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MMG94_RS13915 (MMG94_13865)	2861909..2862316	+	408	WP_016918545.1	type II toxin-antitoxin system VapC family toxin	Toxin
MMG94_RS13920 (MMG94_13870)	2862313..2863479	-	1167	WP_016918544.1	phosphoserine transaminase	-
MMG94_RS13925 (MMG94_13875)	2863632..2864096	-	465	WP_026016048.1	hypothetical protein	-
MMG94_RS13930 (MMG94_13880)	2864250..2864543	+	294	WP_016918542.1	DUF6156 family protein	-
MMG94_RS13935 (MMG94_13885)	2864575..2865546	+	972	WP_016918541.1	alpha/beta fold hydrolase	-
MMG94_RS13940 (MMG94_13890)	2865563..2865859	-	297	WP_016918540.1	BolA family protein	-
MMG94_RS13945 (MMG94_13895)	2865993..2866586	+	594	WP_016918539.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14766.90 Da
• Isoelectric Point: 6.4730

>T237424 WP_016918545.1 NZ_CP092968:2861909-2862316 [Methylocystis parvus OBBP]
MFCLDTNVVIFALNGRRAGYAERLAEEIRAGTKLIVPSVVLFELSYGIAKSRQPEKARALLVEFLSAGFEQPAFDADDAH
EAGDIRAHLEARGAPIGPFDVLIAAQARRRGAVLVTNNRREFDRVPGLMATDWAA

Nucleotide

• Length: 408 bp

>T237424 NZ_LR882052:c4098250-4098077 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9458.76 Da
• Isoelectric Point: 4.6437

>AT237424 WP_016918546.1 NZ_CP092968:2861646-2861900 [Methylocystis parvus OBBP]
MATAKLFQHGRSQAVRLPKEFRLPGTEVRVSKVGDKVILEPLESEPFDVDAWRLRLDALGAKDFLPEGIPDDQPLRPDES
ISFD

Nucleotide

• Length: 255 bp

>AT237424 NZ_LR882052:c4098574-4098278 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A6B8M3R6