Detailed information of TA system TA237423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2721583..2722361
• Replicon: chromosome
• Accession: NZ_CP092968
• Organism: Methylocystis parvus OBBP

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: MMG94_RS13220
• Protein ID: WP_016920155.1
• Coordinates: 2721861..2722361 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A6B8M2P1
• Locus tag: MMG94_RS13215
• Protein ID: WP_016920154.1
• Coordinates: 2721583..2721864 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMG94_RS13195 (MMG94_13150)	2717671..2717889	+	219	WP_016920150.1	YbdD/YjiX family protein	-
MMG94_RS13200 (MMG94_13155)	2718000..2718860	+	861	WP_016920151.1	AraC family transcriptional regulator	-
MMG94_RS13205 (MMG94_13160)	2718981..2719820	+	840	WP_016920152.1	uracil-DNA glycosylase	-
MMG94_RS13210 (MMG94_13165)	2719965..2721260	-	1296	WP_016920153.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MMG94_RS13215 (MMG94_13170)	2721583..2721864	+	282	WP_016920154.1	DUF1778 domain-containing protein	Antitoxin
MMG94_RS13220 (MMG94_13175)	2721861..2722361	+	501	WP_016920155.1	GNAT family N-acetyltransferase	Toxin
MMG94_RS13225 (MMG94_13180)	2722358..2723311	-	954	WP_016920156.1	J domain-containing protein	-
MMG94_RS13230 (MMG94_13185)	2723423..2723713	-	291	WP_016920157.1	hypothetical protein	-
MMG94_RS13235 (MMG94_13190)	2723780..2725030	-	1251	WP_244415352.1	Nramp family divalent metal transporter	-
MMG94_RS13240 (MMG94_13195)	2725132..2726229	-	1098	WP_016920159.1	histidine phosphatase family protein	-
MMG94_RS13245 (MMG94_13200)	2726377..2726631	-	255	WP_016920160.1	hypothetical protein	-
MMG94_RS13250 (MMG94_13205)	2726711..2727262	-	552	WP_016920161.1	TIGR02301 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 17751.48 Da
• Isoelectric Point: 6.9524

>T237423 WP_016920155.1 NZ_CP092968:2721861-2722361 [Methylocystis parvus OBBP]
VTLAPPRPILETDDLSVFDCGSVAMDEWLKKRARPNQASGASRTYVVCNDETIVAYFALASGAVDLAEATNRLKRNMPDP
VPVPVAVLGRLAIDRSWQKQGLGRALAKDAFQRVLAASNAIGIRGMLVHAISAEAAAFYQATGFTPSPLDPMTLMATLID
LRAALD

Nucleotide

• Length: 501 bp

>T237423 NZ_LR882052:c3855711-3855424 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10618.11 Da
• Isoelectric Point: 11.5708

>AT237423 WP_016920154.1 NZ_CP092968:2721583-2721864 [Methylocystis parvus OBBP]
MQSRSNTAKRDTLNLRVKPGDRELIDRAATLVGKSRTDFLLDAGRRAAQDALLDRRLWRVDSAAFDAFVALLDAPPQPNE
RLRRTMKTRAPWE

Nucleotide

• Length: 282 bp

>AT237423 NZ_LR882052:c3855437-3855135 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6B8M2P1