Detailed information of TA system TA237421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 753358..754147
• Replicon: chromosome
• Accession: NZ_CP092968
• Organism: Methylocystis parvus OBBP

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MMG94_RS03725
• Protein ID: WP_016918666.1
• Coordinates: 753641..754147 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: MMG94_RS03720
• Protein ID: WP_016918667.1
• Coordinates: 753358..753648 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMG94_RS03690 (MMG94_03675)	748667..749128	+	462	WP_016918673.1	hypothetical protein	-
MMG94_RS03695 (MMG94_03680)	749139..750011	-	873	WP_016918672.1	methyltransferase domain-containing protein	-
MMG94_RS03700 (MMG94_03685)	750258..750788	+	531	WP_016918671.1	L,D-transpeptidase	-
MMG94_RS03705 (MMG94_03690)	751171..751674	+	504	WP_016918670.1	YbjN domain-containing protein	-
MMG94_RS03710 (MMG94_03695)	751722..752489	+	768	WP_244415223.1	pyrroline-5-carboxylate reductase	-
MMG94_RS03715 (MMG94_03700)	752578..753240	+	663	WP_040578936.1	TetR family transcriptional regulator	-
MMG94_RS03720 (MMG94_03705)	753358..753648	+	291	WP_016918667.1	DUF1778 domain-containing protein	Antitoxin
MMG94_RS03725 (MMG94_03710)	753641..754147	+	507	WP_016918666.1	GNAT family N-acetyltransferase	Toxin
MMG94_RS03730 (MMG94_03715)	754195..755565	-	1371	WP_016918665.1	ATP-binding protein	-
MMG94_RS03735 (MMG94_03720)	755580..756344	-	765	WP_016918664.1	response regulator transcription factor	-
MMG94_RS03740 (MMG94_03725)	756341..756859	-	519	WP_051001081.1	MarR family transcriptional regulator	-
MMG94_RS03745 (MMG94_03730)	757049..757921	+	873	WP_016918662.1	branched-chain amino acid aminotransferase	-
MMG94_RS03750 (MMG94_03735)	758012..758494	-	483	WP_020372401.1	hypothetical protein	-
MMG94_RS03755 (MMG94_03740)	758540..759103	-	564	WP_016918660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (23-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-92)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18258.13 Da
• Isoelectric Point: 9.9196

>T237421 WP_016918666.1 NZ_CP092968:753641-754147 [Methylocystis parvus OBBP]
VNKSSSVEKLHRKHAVEEFTCGRPELDRFLIRHALQAQRMDSSRTYLGLSGEAVIGYHTIVVGQAVHADAPERVSKGMPR
HPIPLLILARLAVHIDWRGRGVGAGLLLDAMGRTLQVAEIAGVHALAVHAKDEAAAAFYAHFGFIPSPTDARHLLMPIKD
IRAAAGEK

Nucleotide

• Length: 507 bp

>T237421 NZ_LR882052:c3538479-3538081 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11113.67 Da
• Isoelectric Point: 6.4102

>AT237421 WP_016918667.1 NZ_CP092968:753358-753648 [Methylocystis parvus OBBP]
MITPRTTRSEKLDLRLTGEAKQMLQEAAAVAQKTVSEFVLESALSEARERLSDRRVFILDEERWVALRAALDAPPRRQPR
LEKLFSEPSFFDPNRE

Nucleotide

• Length: 291 bp

>AT237421 NZ_LR882052:c3538775-3538482 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA