Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 26927..27538 Replicon plasmid pTagA
Accession NZ_CP092924
Organism Pseudomonas syringae pv. tagetis strain ICMP 4091

Toxin (Protein)


Gene name PfiT Uniprot ID A0A0Q0EJP2
Locus tag MME58_RS26955 Protein ID WP_020304269.1
Coordinates 27188..27538 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID Q48B86
Locus tag MME58_RS26950 Protein ID WP_004662018.1
Coordinates 26927..27175 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MME58_RS26930 (MME58_26930) 22156..23052 + 897 WP_003317531.1 LysR family transcriptional regulator -
MME58_RS26935 (MME58_26935) 23210..24202 + 993 WP_003317529.1 YncE family protein -
MME58_RS26940 (MME58_26940) 24532..24912 + 381 WP_003317528.1 hypothetical protein -
MME58_RS26945 (MME58_26945) 25627..26589 - 963 WP_003425065.1 tyrosine-type recombinase/integrase -
MME58_RS26950 (MME58_26950) 26927..27175 + 249 WP_004662018.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
MME58_RS26955 (MME58_26955) 27188..27538 + 351 WP_020304269.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MME58_RS26960 (MME58_26960) 27654..28427 - 774 WP_055005858.1 helix-turn-helix transcriptional regulator -
MME58_RS26965 (MME58_26965) 28536..29636 + 1101 WP_055005859.1 polyamine ABC transporter substrate-binding protein -
MME58_RS26970 (MME58_26970) 29734..29931 + 198 WP_020304272.1 hypothetical protein -
MME58_RS26975 (MME58_26975) 29943..30338 - 396 WP_055005860.1 helix-turn-helix transcriptional regulator -
MME58_RS26980 (MME58_26980) 30555..31067 + 513 WP_055005870.1 transcription termination/antitermination NusG family protein -
MME58_RS26985 (MME58_26985) 31156..31398 + 243 WP_055005861.1 hypothetical protein -
MME58_RS26990 (MME58_26990) 31411..32118 + 708 WP_055005862.1 lytic transglycosylase domain-containing protein -
MME58_RS26995 (MME58_26995) 32118..32453 + 336 WP_025391077.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..56569 56569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13013.80 Da        Isoelectric Point: 4.1824

>T237420 WP_020304269.1 NZ_CP092924:27188-27538 [Pseudomonas syringae pv. tagetis]
MNTFALRFTDVAQQSLEDQVEHLAVYQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDISVLLVLGSKQSVEQALIRYCLLQPM

Download         Length: 351 bp

>T237420 NZ_LR882052:3345858-3346076 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9352.70 Da        Isoelectric Point: 9.8450

>AT237420 WP_004662018.1 NZ_CP092924:26927-27175 [Pseudomonas syringae pv. tagetis]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFA
QK

Download         Length: 249 bp

>AT237420 NZ_LR882052:3345458-3345832 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0EJP2

Antitoxin

Source ID Structure
AlphaFold DB A0A0N8T3U9

References