Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PfiT-PfiA/ParE-Phd |
| Location | 26927..27538 | Replicon | plasmid pTagA |
| Accession | NZ_CP092924 | ||
| Organism | Pseudomonas syringae pv. tagetis strain ICMP 4091 | ||
Toxin (Protein)
| Gene name | PfiT | Uniprot ID | A0A0Q0EJP2 |
| Locus tag | MME58_RS26955 | Protein ID | WP_020304269.1 |
| Coordinates | 27188..27538 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | PfiA | Uniprot ID | Q48B86 |
| Locus tag | MME58_RS26950 | Protein ID | WP_004662018.1 |
| Coordinates | 26927..27175 (+) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MME58_RS26930 (MME58_26930) | 22156..23052 | + | 897 | WP_003317531.1 | LysR family transcriptional regulator | - |
| MME58_RS26935 (MME58_26935) | 23210..24202 | + | 993 | WP_003317529.1 | YncE family protein | - |
| MME58_RS26940 (MME58_26940) | 24532..24912 | + | 381 | WP_003317528.1 | hypothetical protein | - |
| MME58_RS26945 (MME58_26945) | 25627..26589 | - | 963 | WP_003425065.1 | tyrosine-type recombinase/integrase | - |
| MME58_RS26950 (MME58_26950) | 26927..27175 | + | 249 | WP_004662018.1 | type II toxin-antitoxin system prevent-host-death family antitoxin | Antitoxin |
| MME58_RS26955 (MME58_26955) | 27188..27538 | + | 351 | WP_020304269.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| MME58_RS26960 (MME58_26960) | 27654..28427 | - | 774 | WP_055005858.1 | helix-turn-helix transcriptional regulator | - |
| MME58_RS26965 (MME58_26965) | 28536..29636 | + | 1101 | WP_055005859.1 | polyamine ABC transporter substrate-binding protein | - |
| MME58_RS26970 (MME58_26970) | 29734..29931 | + | 198 | WP_020304272.1 | hypothetical protein | - |
| MME58_RS26975 (MME58_26975) | 29943..30338 | - | 396 | WP_055005860.1 | helix-turn-helix transcriptional regulator | - |
| MME58_RS26980 (MME58_26980) | 30555..31067 | + | 513 | WP_055005870.1 | transcription termination/antitermination NusG family protein | - |
| MME58_RS26985 (MME58_26985) | 31156..31398 | + | 243 | WP_055005861.1 | hypothetical protein | - |
| MME58_RS26990 (MME58_26990) | 31411..32118 | + | 708 | WP_055005862.1 | lytic transglycosylase domain-containing protein | - |
| MME58_RS26995 (MME58_26995) | 32118..32453 | + | 336 | WP_025391077.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..56569 | 56569 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13013.80 Da Isoelectric Point: 4.1824
>T237420 WP_020304269.1 NZ_CP092924:27188-27538 [Pseudomonas syringae pv. tagetis]
MNTFALRFTDVAQQSLEDQVEHLAVYQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDISVLLVLGSKQSVEQALIRYCLLQPM
MNTFALRFTDVAQQSLEDQVEHLAVYQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDISVLLVLGSKQSVEQALIRYCLLQPM
Download Length: 351 bp
>T237420 NZ_LR882052:3345858-3346076 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
Antitoxin
Download Length: 83 a.a. Molecular weight: 9352.70 Da Isoelectric Point: 9.8450
>AT237420 WP_004662018.1 NZ_CP092924:26927-27175 [Pseudomonas syringae pv. tagetis]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFA
QK
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFA
QK
Download Length: 249 bp
>AT237420 NZ_LR882052:3345458-3345832 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0Q0EJP2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0N8T3U9 |