Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 780985..781565 Replicon chromosome
Accession NZ_CP092923
Organism Pseudomonas syringae pv. tagetis strain ICMP 4091

Toxin (Protein)


Gene name PumA Uniprot ID A0A0Q0EIR5
Locus tag MME58_RS03455 Protein ID WP_032620802.1
Coordinates 780985..781284 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A2G4CUT8
Locus tag MME58_RS03460 Protein ID WP_003407168.1
Coordinates 781281..781565 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MME58_RS03425 (MME58_03425) 777115..777795 + 681 WP_054986846.1 hypothetical protein -
MME58_RS03430 (MME58_03430) 778241..778426 + 186 WP_221041297.1 phosphoribulokinase -
MME58_RS03435 (MME58_03435) 778663..778914 - 252 WP_003348789.1 ribbon-helix-helix protein, CopG family -
MME58_RS03440 (MME58_03440) 779003..779656 - 654 WP_054986844.1 AAA family ATPase -
MME58_RS03445 (MME58_03445) 780051..780467 - 417 WP_055005949.1 hypothetical protein -
MME58_RS03450 (MME58_03450) 780526..780813 - 288 WP_041924941.1 hypothetical protein -
MME58_RS03455 (MME58_03455) 780985..781284 + 300 WP_032620802.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MME58_RS03460 (MME58_03460) 781281..781565 + 285 WP_003407168.1 putative addiction module antidote protein Antitoxin
MME58_RS03470 (MME58_03470) 781980..782319 - 340 Protein_678 DUF4113 domain-containing protein -
MME58_RS03475 (MME58_03475) 782342..784693 + 2352 WP_169790335.1 Tn3 family transposase -
MME58_RS03480 (MME58_03480) 784792..786015 - 1224 WP_055005951.1 type III secretion system effector HopQ1-1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - pilR 704666..794493 89827
- flank IS/Tn - - 782342..784693 2351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(10-92)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11140.78 Da        Isoelectric Point: 10.1771

>T237419 WP_032620802.1 NZ_CP092923:780985-781284 [Pseudomonas syringae pv. tagetis]
MTTIKQTSTYMAWERRLKDQKAKAAIAARIFRVANGLMGDVSPVGQGVSELRIHVGPGYRVYFQQRGDELILLLCGGDKS
SQSRDIETAKKLADQWRQE

Download         Length: 300 bp

>T237419 NZ_LR882052:2577818-2577925 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10277.83 Da        Isoelectric Point: 5.0916

>AT237419 WP_003407168.1 NZ_CP092923:781281-781565 [Pseudomonas syringae pv. tagetis]
MKEQIFDYDPAAALDGPEAIAIFISDAFETGDAEYIAKAMGVVARAKGMTELARETGLSREQLYRSFSSKGNPTLKTMLT
VMRALGVDMTARMH

Download         Length: 285 bp

>AT237419 NZ_LR882052:c2577771-2577705 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0EIR5

Antitoxin

Source ID Structure
AlphaFold DB A0A2G4CUT8

References