Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 634566..636295 Replicon chromosome
Accession NZ_CP092923
Organism Pseudomonas syringae pv. tagetis strain ICMP 4091

Toxin (Protein)


Gene name pmenT Uniprot ID A0A0N8T227
Locus tag MME58_RS02755 Protein ID WP_054987015.1
Coordinates 635651..636295 (-) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A0P9R7I4
Locus tag MME58_RS02750 Protein ID WP_054987014.1
Coordinates 634566..635654 (-) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MME58_RS02720 (MME58_02720) 629678..629998 + 321 WP_055007006.1 hypothetical protein -
MME58_RS02725 (MME58_02725) 629998..631773 + 1776 WP_055007005.1 hypothetical protein -
MME58_RS02730 (MME58_02730) 631770..632375 + 606 WP_055007004.1 hypothetical protein -
MME58_RS02735 (MME58_02735) 632430..633473 + 1044 WP_055007003.1 WavE lipopolysaccharide synthesis family protein -
MME58_RS02740 (MME58_02740) 633503..633775 - 273 WP_055007002.1 helix-turn-helix transcriptional regulator -
MME58_RS02745 (MME58_02745) 633907..634272 + 366 WP_055007016.1 hypothetical protein -
MME58_RS02750 (MME58_02750) 634566..635654 - 1089 WP_054987014.1 macro domain-containing protein Antitoxin
MME58_RS02755 (MME58_02755) 635651..636295 - 645 WP_054987015.1 DUF4433 domain-containing protein Toxin
MME58_RS02760 (MME58_02760) 636292..636873 - 582 WP_055007001.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
MME58_RS02765 (MME58_02765) 637751..638482 - 732 WP_055007000.1 XRE family transcriptional regulator -
MME58_RS02770 (MME58_02770) 638841..639368 - 528 WP_054984741.1 inorganic diphosphatase -
MME58_RS02775 (MME58_02775) 639479..640288 - 810 WP_054984742.1 zinc-dependent peptidase -
MME58_RS02780 (MME58_02780) 640292..640939 - 648 WP_055006998.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24634.19 Da        Isoelectric Point: 7.5091

>T237418 WP_054987015.1 NZ_CP092923:c636295-635651 [Pseudomonas syringae pv. tagetis]
MKYSSLNPKKALIWRIVHRDNLPWILDNGLHCASSDVQAPQYVNIGNLDLIGKRRNRGVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGRSVQQRSNDEIVILVSSLPRVEQLGLAFVFTNAHAYPDWTDYYSELASLDQIDWSIIQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

>T237418 NZ_LR882052:2577283-2577390 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40454.46 Da        Isoelectric Point: 6.5081

>AT237418 WP_054987014.1 NZ_CP092923:c635654-634566 [Pseudomonas syringae pv. tagetis]
MIRFTQGNLLKADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNQVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKHSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGINDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLSKSGVSPTVPAVREA
LKNWDGGAGAAARKSRLFDDQALGIALKRLTASNLSPEIAVP

Download         Length: 1089 bp

>AT237418 NZ_LR882052:c2577236-2577170 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0N8T227

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9R7I4

References